Cloned (Comment) | Organism |
---|---|
cloned into the bacterial expression vector pGEX-2T and used to transform Escherichia coli strain pGEX-2T | Nicotiana tabacum |
wild-type recombinant enzyme fused with glutathione S-transferase | Nicotiana tabacum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
metsulfuron-methyl | - |
Nicotiana tabacum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.1 | - |
pyruvate | wild-type recombinant enzyme | Nicotiana tabacum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate | Nicotiana tabacum | first enzyme unique to biosynthesis of the branched chain amino acids Val, Leu, and Ile | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Nicotiana tabacum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate | - |
Nicotiana tabacum | 2-acetolactate + CO2 | - |
? | |
pyruvate | first enzyme unique to biosynthesis of the branched chain amino acids Val, Leu, and Ile | Nicotiana tabacum | ? | - |
? |