KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
2-oxoglutarate | - |
Rattus norvegicus | |
6.8 | - |
glyoxylate | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | part of alpha-ketoglutarate dehydrogenase complex | Rattus norvegicus | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + glyoxylate | Rattus norvegicus | important as diversion of glyoxylate from oxalate formation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
rat | - |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.1 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-20°C, potassium phosphate buffer, pH 7.1, 10% glycerol, 1 mM dithiothreitol, at least several days | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + glyoxylate | - |
Rattus norvegicus | 2-hydroxy-3-oxohexandioate + CO2 | - |
? | |
2-oxoglutarate + glyoxylate | important as diversion of glyoxylate from oxalate formation | Rattus norvegicus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
enzyme assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
enzyme assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Rattus norvegicus |