Cloned (Comment) | Organism |
---|---|
gene POMT7, error-prone polymerase chain reaction, expression of GST-tagged wild-type POMT7 and mutant POMT7-M1 in Escherichia coli | Populus sp. |
Protein Variants | Comment | Organism |
---|---|---|
D257G | random mutagenesis, the mutant of POMT-7, POMT7-M1, has a novel regioselectivity, from large scale mutant screening, mutant D257G methylates the 3-hydroxyl group of flavonols in addition to 7-hydrdoxyl group | Populus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0298 | - |
kaempferol | pH 7.5, 37°C, recombinant wild-type POMT7 | Populus sp. | |
0.0324 | - |
kaempferol | pH 7.5, 37°C, recombinant mutant POMT7-M1 | Populus sp. | |
0.0324 | - |
quercetin | pH 7.5, 37°C, recombinant wild-type POMT7 | Populus sp. | |
0.0378 | - |
quercetin | pH 7.5, 37°C, recombinant mutant POMT7-M1 | Populus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + kaempferol | Populus sp. | - |
S-adenosyl-L-homocysteine + 7-O-methylkaempferol | - |
? | |
S-adenosyl-L-methionine + quercetin | Populus sp. | - |
S-adenosyl-L-homocysteine + 7-O-methylquercetin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Populus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged wild-type POMT7 and mutant POMT7-M1 fromEscherichia coli by glutathione affinity chromatography | Populus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + kaempferol | activity only of enzyme mutant POMT7-M1, D257G, not of wild-type POMT7 | Populus sp. | 2 S-adenosyl-L-homocysteine + 3,7-O-dimethylkaempferol | - |
? | |
2 S-adenosyl-L-methionine + quercetin | activity only of enzyme mutant POMT7-M1, D257G, not of wild-type POMT7, molecular docking of quercetin into the modeled structure of Asp257Gly | Populus sp. | 2 S-adenosyl-L-homocysteine + 3,7-O-dimethylquercetin | - |
? | |
S-adenosyl-L-methionine + kaempferol | - |
Populus sp. | S-adenosyl-L-homocysteine + 7-O-methylkaempferol | - |
? | |
S-adenosyl-L-methionine + quercetin | - |
Populus sp. | S-adenosyl-L-homocysteine + 7-O-methylquercetin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
flavonoid O-methyltransferase | - |
Populus sp. |
POMT7 | - |
Populus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Populus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.064 | - |
kaempferol | pH 7.5, 37°C, recombinant mutant POMT7-M1 | Populus sp. | |
0.064 | - |
quercetin | pH 7.5, 37°C, recombinant wild-type POMT7 | Populus sp. | |
0.07 | - |
quercetin | pH 7.5, 37°C, recombinant mutant POMT7-M1 | Populus sp. | |
0.071 | - |
kaempferol | pH 7.5, 37°C, recombinant wild-type POMT7 | Populus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Populus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Populus sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.7 | - |
quercetin | pH 7.5, 37°C, recombinant mutant POMT7-M1 | Populus sp. | |
1.9 | - |
kaempferol | pH 7.5, 37°C, recombinant mutant POMT7-M1 | Populus sp. | |
1.9 | - |
quercetin | pH 7.5, 37°C, recombinant wild-type POMT7 | Populus sp. | |
2.4 | - |
kaempferol | pH 7.5, 37°C, recombinant wild-type POMT7 | Populus sp. |