General Stability | Organism |
---|---|
no stabilization by glycerol, 10-40% v/v, or nicotinic acid | Glycine max |
sulfhydryl group protecting agents required for stabilization during purification | Glycine max |
total loss of activity by freezing at -20°C | Glycine max |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | - |
Glycine max | |
Mg2+ | slight | Glycine max | |
p-hydroxymercuribenzoate | - |
Glycine max | |
S-adenosyl-L-homocysteine | - |
Glycine max | |
SH-blocking reagents | - |
Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.055 | - |
S-adenosyl-L-methionine | - |
Glycine max | |
0.078 | - |
nicotinate | - |
Glycine max |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement for divalent cations | Glycine max |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
ultracentrifugation, gel filtration | Glycine max |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + nicotinate | Glycine max | - |
S-adenosyl-L-homocysteine + 1-methylnicotinate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
soybean | - |
Oxidation Stability | Organism |
---|---|
rigorous exclusion of O2 by saturating the buffer with N2 allows partial stabilization during purification | Glycine max |
Purification (Comment) | Organism |
---|---|
- |
Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | heterotrophic | Glycine max | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Glycine max |
Storage Stability | Organism |
---|---|
4°C, 24 h, N2-atmosphere | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + nicotinate | - |
Glycine max | S-adenosyl-L-homocysteine + 1-methylnicotinate | - |
? | |
S-adenosyl-L-methionine + nicotinate | high specificity for nicotinate, no other pyridine derivative is a suitable methyl group acceptor | Glycine max | S-adenosyl-L-homocysteine + 1-methylnicotinate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 40 | - |
Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Glycine max |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | pH 5.0: about 50% of maximum activity, pH 9.0: nearly inactive | Glycine max |