Protein Variants | Comment | Organism |
---|---|---|
D133A | complete loss of activity | Aquifex aeolicus |
D133N | complete loss of activity | Aquifex aeolicus |
D74A | 21% of wild-type activity | Aquifex aeolicus |
D97A | 21% of wild-type activity | Aquifex aeolicus |
E168A | 100% of wild-type activity | Aquifex aeolicus |
E47A | 0.4% of wild-type activity | Aquifex aeolicus |
H104A | 140% of wild-type activity | Aquifex aeolicus |
K101A | 36% of wild-type activity | Aquifex aeolicus |
R107A | 140% of wild-type activity | Aquifex aeolicus |
R108A | 2% of wild-type activity | Aquifex aeolicus |
T132A | 70% of wild-type activity | Aquifex aeolicus |
T132C | 18% of wild-type activity | Aquifex aeolicus |
T132S | 27% of wild-type activity | Aquifex aeolicus |
T165A | 7% of wild-type activity | Aquifex aeolicus |
Y167A | 0.2% of wild-type activity | Aquifex aeolicus |
Y167F | 12% of wild-type activity | Aquifex aeolicus |
Y95A | 2% of wild-type activity | Aquifex aeolicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
S-adenosyl-L-methionine | mutant D97A, 55°C, pH not specified in the publication | Aquifex aeolicus | |
0.0007 | - |
S-adenosyl-L-methionine | wild-type, 55°C, pH not specified in the publication | Aquifex aeolicus | |
0.0008 | - |
S-adenosyl-L-methionine | mutant D74A, 55°C, pH not specified in the publication | Aquifex aeolicus | |
0.001 | - |
S-adenosyl-L-methionine | mutant T132S, 55°C, pH not specified in the publication | Aquifex aeolicus | |
0.0017 | - |
S-adenosyl-L-methionine | mutant T132A, 55°C, pH not specified in the publication | Aquifex aeolicus | |
0.0025 | - |
S-adenosyl-L-methionine | mutant H104A, 55°C, pH not specified in the publication | Aquifex aeolicus | |
0.0025 | - |
S-adenosyl-L-methionine | mutant R107A, 55°C, pH not specified in the publication | Aquifex aeolicus | |
0.003 | - |
S-adenosyl-L-methionine | mutant K101A, 55°C, pH not specified in the publication | Aquifex aeolicus | |
0.003 | - |
S-adenosyl-L-methionine | mutant T132C, 55°C, pH not specified in the publication | Aquifex aeolicus | |
0.005 | - |
S-adenosyl-L-methionine | mutant T165A, 55°C, pH not specified in the publication | Aquifex aeolicus | |
0.006 | - |
S-adenosyl-L-methionine | mutant Y167F, 55°C, pH not specified in the publication | Aquifex aeolicus | |
0.007 | - |
S-adenosyl-L-methionine | mutant E168A, 55°C, pH not specified in the publication | Aquifex aeolicus | |
0.007 | - |
S-adenosyl-L-methionine | mutant E47A, 55°C, pH not specified in the publication | Aquifex aeolicus | |
0.0075 | - |
S-adenosyl-L-methionine | mutant Y95A, 55°C, pH not specified in the publication | Aquifex aeolicus | |
0.01 | - |
S-adenosyl-L-methionine | mutant R108A, 55°C, pH not specified in the publication | Aquifex aeolicus | |
0.019 | - |
S-adenosyl-L-methionine | mutant Y167A, 55°C, pH not specified in the publication | Aquifex aeolicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | O66479 | - |
- |
Purification (Comment) | Organism |
---|---|
purification method to remove contaminating RNA completely by using 6 M urea | Aquifex aeolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine46 in tRNAPhe | substrate tRNAPhe of Saccharomyces cerevisiae | Aquifex aeolicus | S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNAPhe | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TrmB | - |
Aquifex aeolicus |
General Information | Comment | Organism |
---|---|---|
physiological function | residues Glu47, Tyr95, Arg108, Thr165 and Tyr167 residues are important for AdoMet binding and Asp74, Asp97, and Thr132 are important for the methyltransfer reaction. During the reaction, the carboxyl group in Asp133 captures the proton of N-H in the guanine base. Thr132 may assist the interaction by formation of a hydrogen bond between the OH group in Thr132 and the O6 atom in the guanine base. Interactions between Thr132, Asp133 and tRNA decide the direction of the guanine base and the N7 atom in G46 causes the nucleophilic attack against the activated methyl group in the bound AdoMet | Aquifex aeolicus |