Protein Variants | Comment | Organism |
---|---|---|
additional information | the m.4435A->G mutation introduces an m1G37 modification of tRNAMet, altering its structure and function. Primer extension and methylation activity assays indeed confirm that the m.4435A3G mutation creates a tRNA methyltransferase 5 (TRMT5)-catalyzed m1G37 modification of tRNAMet | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine37 in tRNA | Methanocaldococcus jannaschii | - |
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine37 in tRNA | Methanocaldococcus jannaschii NBRC 100440 | - |
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine37 in tRNA | Methanocaldococcus jannaschii DSM 2661 | - |
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine37 in tRNA | Methanocaldococcus jannaschii ATCC 43067 | - |
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine37 in tRNA | Methanocaldococcus jannaschii JAL-1 | - |
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine37 in tRNA | Methanocaldococcus jannaschii JCM 10045 | - |
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q58293 | i.e. Methanococcus jannaschii | - |
Methanocaldococcus jannaschii ATCC 43067 | Q58293 | i.e. Methanococcus jannaschii | - |
Methanocaldococcus jannaschii DSM 2661 | Q58293 | i.e. Methanococcus jannaschii | - |
Methanocaldococcus jannaschii JAL-1 | Q58293 | i.e. Methanococcus jannaschii | - |
Methanocaldococcus jannaschii JCM 10045 | Q58293 | i.e. Methanococcus jannaschii | - |
Methanocaldococcus jannaschii NBRC 100440 | Q58293 | i.e. Methanococcus jannaschii | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the mutant tRNAMet transcripts (G37) are modified with m1G37 modification by the Mj-Trm5 but as less efficiently as cytoplasmic tRNALeu(CAG) transcripts. In contrast, the modification is not detected in the human wild-type tRNAMet transcripts (A37) in the presence of Mj-Trm5. The human cytoplasmic tRNALeu(CAG) transcripts (G37) are modified by the Mj-Trm5, whereas human cytoplasmic tRNAThr transcripts (A37) are not modified in the presence of Mj-Trm5. Marked decrease in the steady-state levels of mutated tRNAMet | Methanocaldococcus jannaschii | ? | - |
- |
|
additional information | the mutant tRNAMet transcripts (G37) are modified with m1G37 modification by the Mj-Trm5 but as less efficiently as cytoplasmic tRNALeu(CAG) transcripts. In contrast, the modification is not detected in the human wild-type tRNAMet transcripts (A37) in the presence of Mj-Trm5. The human cytoplasmic tRNALeu(CAG) transcripts (G37) are modified by the Mj-Trm5, whereas human cytoplasmic tRNAThr transcripts (A37) are not modified in the presence of Mj-Trm5. Marked decrease in the steady-state levels of mutated tRNAMet | Methanocaldococcus jannaschii NBRC 100440 | ? | - |
- |
|
additional information | the mutant tRNAMet transcripts (G37) are modified with m1G37 modification by the Mj-Trm5 but as less efficiently as cytoplasmic tRNALeu(CAG) transcripts. In contrast, the modification is not detected in the human wild-type tRNAMet transcripts (A37) in the presence of Mj-Trm5. The human cytoplasmic tRNALeu(CAG) transcripts (G37) are modified by the Mj-Trm5, whereas human cytoplasmic tRNAThr transcripts (A37) are not modified in the presence of Mj-Trm5. Marked decrease in the steady-state levels of mutated tRNAMet | Methanocaldococcus jannaschii DSM 2661 | ? | - |
- |
|
additional information | the mutant tRNAMet transcripts (G37) are modified with m1G37 modification by the Mj-Trm5 but as less efficiently as cytoplasmic tRNALeu(CAG) transcripts. In contrast, the modification is not detected in the human wild-type tRNAMet transcripts (A37) in the presence of Mj-Trm5. The human cytoplasmic tRNALeu(CAG) transcripts (G37) are modified by the Mj-Trm5, whereas human cytoplasmic tRNAThr transcripts (A37) are not modified in the presence of Mj-Trm5. Marked decrease in the steady-state levels of mutated tRNAMet | Methanocaldococcus jannaschii ATCC 43067 | ? | - |
- |
|
additional information | the mutant tRNAMet transcripts (G37) are modified with m1G37 modification by the Mj-Trm5 but as less efficiently as cytoplasmic tRNALeu(CAG) transcripts. In contrast, the modification is not detected in the human wild-type tRNAMet transcripts (A37) in the presence of Mj-Trm5. The human cytoplasmic tRNALeu(CAG) transcripts (G37) are modified by the Mj-Trm5, whereas human cytoplasmic tRNAThr transcripts (A37) are not modified in the presence of Mj-Trm5. Marked decrease in the steady-state levels of mutated tRNAMet | Methanocaldococcus jannaschii JAL-1 | ? | - |
- |
|
additional information | the mutant tRNAMet transcripts (G37) are modified with m1G37 modification by the Mj-Trm5 but as less efficiently as cytoplasmic tRNALeu(CAG) transcripts. In contrast, the modification is not detected in the human wild-type tRNAMet transcripts (A37) in the presence of Mj-Trm5. The human cytoplasmic tRNALeu(CAG) transcripts (G37) are modified by the Mj-Trm5, whereas human cytoplasmic tRNAThr transcripts (A37) are not modified in the presence of Mj-Trm5. Marked decrease in the steady-state levels of mutated tRNAMet | Methanocaldococcus jannaschii JCM 10045 | ? | - |
- |
|
S-adenosyl-L-methionine + guanine37 in tRNA | - |
Methanocaldococcus jannaschii | S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine37 in tRNA | - |
Methanocaldococcus jannaschii NBRC 100440 | S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine37 in tRNA | - |
Methanocaldococcus jannaschii DSM 2661 | S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine37 in tRNA | - |
Methanocaldococcus jannaschii ATCC 43067 | S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine37 in tRNA | - |
Methanocaldococcus jannaschii JAL-1 | S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine37 in tRNA | - |
Methanocaldococcus jannaschii JCM 10045 | S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Mj-Trm5 | - |
Methanocaldococcus jannaschii |
TRM5 | - |
Methanocaldococcus jannaschii |
TRMT5 | - |
Methanocaldococcus jannaschii |
tRNA (m1G37)-methyltransferase | - |
Methanocaldococcus jannaschii |
tRNA methyltransferase 5 | - |
Methanocaldococcus jannaschii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Methanocaldococcus jannaschii |
General Information | Comment | Organism |
---|---|---|
metabolism | a hypertension-associated mitochondrial DNA mutation introduces an m1G37 mutation 4435A->G into human mitochondrial tRNAMet, altering its structure and function, phenotype and pathogenic molecular mechanism, overview. The mutation affects a highly conserved adenosine at position 37, 3' adjacent to the tRNA's anticodon, which is important for the fidelity of codon recognition and stabilization. Defective nucleotide modifications of mitochondrial tRNAs are associated with several human diseases. Trm5 is one of the tRNA (m1G37)-methyltransferases that catalyzes the identical tRNA modification, m1G37 | Methanocaldococcus jannaschii |