Crystallization (Comment) | Organism |
---|---|
analysis of the crystal structure of the Trm5-tRNA-AdoMet ternary complex | Methanocaldococcus jannaschii |
Protein Variants | Comment | Organism |
---|---|---|
D223A | site-directed mutagenesis, the mutant shows altered single turnover kinetics compared to the wild-type enzyme | Methanocaldococcus jannaschii |
D223E | site-directed mutagenesis, the mutant shows altered single turnover kinetics compared to the wild-type enzyme | Methanocaldococcus jannaschii |
D223L | site-directed mutagenesis, the mutant shows complete loss of activity | Methanocaldococcus jannaschii |
D223N | site-directed mutagenesis, the mutant shows complete loss of activity | Methanocaldococcus jannaschii |
E185A | site-directed mutagenesis, the mutant shows complete loss of activity | Methanocaldococcus jannaschii |
E185D | site-directed mutagenesis, the mutant shows altered single turnover kinetics compared to the wild-type enzyme | Methanocaldococcus jannaschii |
E185Q | site-directed mutagenesis, the mutant shows altered single turnover kinetics compared to the wild-type enzyme | Methanocaldococcus jannaschii |
K137A | site-directed mutagenesis, the mutant shows altered single turnover kinetics compared to the wild-type enzyme | Methanocaldococcus jannaschii |
K318A | site-directed mutagenesis, the mutant shows altered single turnover kinetics compared to the wild-type enzyme | Methanocaldococcus jannaschii |
N265A | site-directed mutagenesis, the mutant shows altered single turnover kinetics compared to the wild-type enzyme | Methanocaldococcus jannaschii |
N265H | site-directed mutagenesis, the mutant shows altered single turnover kinetics compared to the wild-type enzyme | Methanocaldococcus jannaschii |
N265Q | site-directed mutagenesis, the mutant shows altered single turnover kinetics compared to the wild-type enzyme | Methanocaldococcus jannaschii |
P267A | site-directed mutagenesis, the mutant shows altered single turnover kinetics compared to the wild-type enzyme | Methanocaldococcus jannaschii |
R145A | site-directed mutagenesis, the mutant shows altered single turnover kinetics compared to the wild-type enzyme | Methanocaldococcus jannaschii |
R181A | site-directed mutagenesis, the mutant shows altered single turnover kinetics compared to the wild-type enzyme | Methanocaldococcus jannaschii |
R186A | site-directed mutagenesis, the mutant shows altered single turnover kinetics compared to the wild-type enzyme | Methanocaldococcus jannaschii |
Y177A | site-directed mutagenesis, the mutant shows altered single turnover kinetics compared to the wild-type enzyme | Methanocaldococcus jannaschii |
Y177F | site-directed mutagenesis, the mutant shows altered single turnover kinetics compared to the wild-type enzyme | Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | wild-type and mutant enzymes pH-dependence of the single-turnover rate constant: the pH dependence of kobs in single-turnover analysis corresponds to proton ransfer during a slower process of induced fit, rather than the bond-breaking and bond-forming steps of methyl transfer, detailed analysis and overview | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine37 in tRNA | Methanocaldococcus jannaschii | - |
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q58293 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + guanine37 in tRNA = S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | mechanism of N-methylation by the tRNA m1G37 methyltransferase Trm5 involving proton abstraction during docking of G37 in the active site by a general base , E185 | Methanocaldococcus jannaschii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | 85°C is the optimal growth temperature | Methanocaldococcus jannaschii | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | structure of Trm5 active site bound to tRNA and S-adenosyl-L-methionine, induced fit for active-site assembly, detailed overview. E185 is crucial both for general base catalysis and for the conformational change that precedes catalysis | Methanocaldococcus jannaschii | ? | - |
? | |
S-adenosyl-L-methionine + guanine37 in tRNA | - |
Methanocaldococcus jannaschii | S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine37 in tRNACys | Methanococcus jannaschii tRNACys | Methanocaldococcus jannaschii | S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNACys | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TRM5 | - |
Methanocaldococcus jannaschii |
tRNA m1G37 methyltransferase | - |
Methanocaldococcus jannaschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
assay at | Methanocaldococcus jannaschii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9.8 | preincubation of Trm5 at pH 6.0, followed by adjustment of the solution to pH 8.0, does not cause irreversible inactivation of the enzyme. Enzyme treated in this manner retained equivalent activity in single-turnover assay | Methanocaldococcus jannaschii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Methanocaldococcus jannaschii |