Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | yield of G10 dimethylation in transcript of tRNAAsp greatly depends on the presence of Mg2+, with an optimal concentration around 5 mM. In contrast, formation of N2-monomethylguanine10 is not strictly dependent on the presence of Mg2+ | Pyrococcus abyssi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus abyssi | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + guanine10 in tRNA | identity elements required for the efficient formation of N2-dimethylguanine10: uracil25 and a V-loop of four nucleotides are major determinants for the formation of N2-dimethylguanine10, whereas the identity of the 13-22 base-pair clearly influences the rate of N2-dimethylguanine10 formation. The integrity of the T-arm prolonged with the amino acid acceptor stem is an important structural element for the dimethylation step catalysed by PabTrm-m2 2G10 enzyme | Pyrococcus abyssi | 2 S-adenosyl-L-homocysteine + N2-dimethylguanine10 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine10 in tRNA | - |
Pyrococcus abyssi | S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PabTrm-m2 2G10 enzyme | - |
Pyrococcus abyssi |