Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Pyrococcus abyssi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
1 * 39000, SDS-PAGE | Pyrococcus abyssi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + guanine10 in tRNA | Pyrococcus abyssi | depending on the experimental conditions used, as well as the tRNA substrate tested, the enzymatic reaction leads to the formation of either N2-methyl or N2-dimethylguanosine. N2-Dimethylguanine10 accumulates during incubation at 50°C, whereas the formation of N2-methylguanine10 appears to be a transient intermediate. At 30°C, N2-dimethylguanine10 is efficiently formed, whereas the formation of N2-methylguanine10 is considerably slowed compared with that at higher temperatures | 2 S-adenosyl-L-homocysteine + N2-dimethylguanine10 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine10 in tRNA | Pyrococcus abyssi | depending on the experimental conditions used, as well as the tRNA substrate tested, the enzymatic reaction leads to the formation of either N2-methyl or N2-dimethylguanosine. N2-Dimethylguanine10 accumulates during incubation at 50°C, whereas the formation of N2-methylguanine10 appears to be a transient intermediate. At 30°C, N2-dimethylguanine10 is efficiently formed, whereas the formation of N2-methylguanine10 is considerably slowed compared with that at higher temperatures | S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus abyssi | Q9UY84 | strain GE5 | - |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus abyssi |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + guanine10 in tRNA = S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA | (1a) | Pyrococcus abyssi | |
S-adenosyl-L-methionine + N2-methylguanine10 in tRNA = S-adenosyl-L-homocysteine + N2-dimethylguanine10 in tRNA | (1b) | Pyrococcus abyssi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + guanine10 in tRNA | depending on the experimental conditions used, as well as the tRNA substrate tested, the enzymatic reaction leads to the formation of either N2-methyl or N2-dimethylguanosine. N2-Dimethylguanine10 accumulates during incubation at 50°C, whereas the formation of N2-methylguanine10 appears to be a transient intermediate. At 30°C, N2-dimethylguanine10 is efficiently formed, whereas the formation of N2-methylguanine10 is considerably slowed compared with that at higher temperatures | Pyrococcus abyssi | 2 S-adenosyl-L-homocysteine + N2-dimethylguanine10 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine10 in tRNA | depending on the experimental conditions used, as well as the tRNA substrate tested, the enzymatic reaction leads to the formation of either N2-methyl or N2-dimethylguanosine. N2-Dimethylguanine10 accumulates during incubation at 50°C, whereas the formation of N2-methylguanine10 appears to be a transient intermediate. At 30°C, N2-dimethylguanine10 is efficiently formed, whereas the formation of N2-methylguanine10 is considerably slowed compared with that at higher temperatures | Pyrococcus abyssi | S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 39000, SDS-PAGE | Pyrococcus abyssi |
Synonyms | Comment | Organism |
---|---|---|
N(2),N(2)-dimethylguanosine tRNA methyltransferase | - |
Pyrococcus abyssi |
PAB1283 | - |
Pyrococcus abyssi |
PabTrm-G10 | - |
Pyrococcus abyssi |
Trm-G10 | - |
Pyrococcus abyssi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Pyrococcus abyssi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
86 | - |
noticeable unfolding of the protein (more than 5%) is detected above 86°C | Pyrococcus abyssi |
102 | - |
midpoint melting temperature | Pyrococcus abyssi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Pyrococcus abyssi |