General Stability | Organism |
---|---|
2-mercaptoethanol stabilizes during purification procedures or storage | Avena sativa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
7-O-methylvitexin | competitive inhibition | Avena sativa | |
7-O-methylvitexin 2''-O-beta-L-rhamnoside | product inhibition, kinetics | Avena sativa | |
additional information | not inhibited by Mg2+ or 2-mercaptoethanol | Avena sativa | |
S-adenosyl-L-homocysteine | strong product inhibition, kinetics | Avena sativa | |
vitexin | competitive inhibition | Avena sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic mechanism, mono-iso Theorell-Chance mechanism with the nucleotide substrate binding before the flavonoid | Avena sativa | |
0.0016 | - |
S-adenosyl-L-methionine | pH 7.5, 30°C | Avena sativa | |
0.015 | - |
vitexin 2''-O-beta-L-rhamnoside | pH 7.5, 30°C | Avena sativa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not activated by Mg2+ or 2-mercaptoethanol | Avena sativa | |
phosphate | the highest activity is obtained in 0.1-0.2 M phosphate buffer, at lower ionic strength the activity drops drastically | Avena sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
gel filtration | Avena sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + vitexin 2''-O-beta-L-rhamnoside | Avena sativa | last step in the biosynthetic pathway to the flavonoid 7-O-methylvitexin 2-O-rhamnoside | S-adenosyl-L-homocysteine + 7-O-methylvitexin 2''-O-beta-L-rhamnoside | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Avena sativa | - |
Gelbhafer Flämingskrone | - |
Purification (Comment) | Organism |
---|---|
180fold | Avena sativa |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + vitexin 2''-O-beta-L-rhamnoside = S-adenosyl-L-homocysteine + 7-O-methylvitexin 2''-O-beta-L-rhamnoside + H+ | the flavonoids vitexin and isovitexin 2-O-arabinoside do not act as substrates for the enzyme from oats, Avena sativa, kinetic mechanism, mono-iso Theorell-Chance mechanism | Avena sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | primary leaves | Avena sativa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Avena sativa |
Storage Stability | Organism |
---|---|
-20°C, purified enzyme, 10 mM 2-mercaptoethanol, 50% glycerol, 1 year, 30-40% loss of activity | Avena sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | not: caffeic acid, naringenin, vitexin, isovitexin 2-O-arabinoside | Avena sativa | ? | - |
? | |
S-adenosyl-L-methionine + apigenin | 5-10% of the rate with vitexin 2-O-rhamnoside as substrate | Avena sativa | S-adenosyl-L-homocysteine + 7-O-methylapigenin | - |
? | |
S-adenosyl-L-methionine + vitexin 2''-O-beta-L-rhamnoside | last step in the biosynthetic pathway to the flavonoid 7-O-methylvitexin 2-O-rhamnoside | Avena sativa | S-adenosyl-L-homocysteine + 7-O-methylvitexin 2''-O-beta-L-rhamnoside | - |
? | |
S-adenosyl-L-methionine + vitexin 2''-O-beta-L-rhamnoside | catalyzes the transfer of the methyl group of S-adenosyl-L-methionine to the A-ring 7-hydroxyl group of vitexin 2-O-rhamnoside, very high substrate specificity, mono-iso Theorell-Chance mechanism with the nucleotide substrate binding before the flavonoid | Avena sativa | S-adenosyl-L-homocysteine + 7-O-methylvitexin 2''-O-beta-L-rhamnoside | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AdoMet:vitexin 2''-O-rhamnoside 7-O-methyltransferase | - |
Avena sativa |
methyltransferase, vitexin 2''-O-rhamnoside 7-O- | - |
Avena sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Avena sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Avena sativa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.4 | 8.6 | 50% of the maximum activity at pH 6.4 and 8.6 | Avena sativa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Avena sativa |