Literature summary for 1.97.1.1 extracted from

Hellberg Lindqvist, M.; Nilsson, T.; Sundin, P.; Rova, M.
Chlorate reductase is cotranscribed with cytochrome c and other downstream genes in the gene cluster for chlorate respiration of Ideonella dechloratans (2015), FEMS Microbiol. Lett., 362, fnv019 .

Cloned(Commentary)

Cloned (Comment)	Organism
gene cluster clrABDC, genetic organization, chlorate reductase is cotranscribed with cytochrome c, UniProt ID B3VLA9, and other downstream genes in the gene cluster for chlorate respiration of Ideonella dechloratans. In vivo production of a single polycistronic transcript covering eight open reading frames including clrABDC, cyc, mobB and arsR. Transcription levels of the cyc and clrA genes are compared to each other by the use of qRT-PCR in RNA preparations from cells grown under aerobic or chlorate reducing anaerobic conditions. The two genes show the same mRNA levels under both growth regimes, indicating that no transcription termination occurs between them. Higher transcription levels are observed at growth without external oxygen supply. Quantitative real-time RT-PCR expression analysis	Ideonella dechloratans

Cloned (Comment)

Organism

gene cluster clrABDC, genetic organization, chlorate reductase is cotranscribed with cytochrome c, UniProt ID B3VLA9, and other downstream genes in the gene cluster for chlorate respiration of Ideonella dechloratans. In vivo production of a single polycistronic transcript covering eight open reading frames including clrABDC, cyc, mobB and arsR. Transcription levels of the cyc and clrA genes are compared to each other by the use of qRT-PCR in RNA preparations from cells grown under aerobic or chlorate reducing anaerobic conditions. The two genes show the same mRNA levels under both growth regimes, indicating that no transcription termination occurs between them. Higher transcription levels are observed at growth without external oxygen supply. Quantitative real-time RT-PCR expression analysis

Ideonella dechloratans

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	in cytochrome c	Ideonella dechloratans

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
reduced acceptor + chlorate	Ideonella dechloratans	-	acceptor + H2O + chlorite	-	?

Organism

Organism	UniProt	Comment	Textmining
Ideonella dechloratans	P60068 AND P60069 AND P60000	subunits alpha, beta, and gamma	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
ferricytochrome c + chlorate	-	Ideonella dechloratans	ferrocytochrome c + H2O + chlorite	-	?
additional information	a c-type cytochrome from Ideonella dechloratans donates electrons to Clr in vitro	Ideonella dechloratans	?	-	?
reduced acceptor + chlorate	-	Ideonella dechloratans	acceptor + H2O + chlorite	-	?

Synonyms

Synonyms	Comment	Organism
clr	-	Ideonella dechloratans

Cofactor

Cofactor	Comment	Organism	Structure
cytochrome c	-	Ideonella dechloratans

General Information

General Information	Comment	Organism
additional information	the genes for the enzymes chlorate reductase (clrABDC) and chlorite dismutase, necessary for chlorate metabolism and probably acquired by lateral gene transfer, are located in a gene cluster that also includes other genes potentially important for chlorate metabolism. Among those are a gene for cytochrome c (cyc) whose gene product may serve as an electron carrier during chlorate reduction, a cofactor biosynthesis gene (mobB) and a predicted transcriptional regulator (arsR)	Ideonella dechloratans
physiological function	a soluble periplasmic c-type cytochrome from Ideonella dechloratans donates electrons to Clr in vitro	Ideonella dechloratans