Cloned (Comment) | Organism |
---|---|
structural genes, napA and napB, are cloned, and their nucleotide sequences is determined | Cupriavidus necator |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the physiological role of NAP in nitrate metabolism is investigated in studies with a mutant bearing a transposon insertion in one of the structural genes | Cupriavidus necator |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
nitrate | pH 5.5, 37°C | Cupriavidus necator |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Cupriavidus necator | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17000 | - |
1 * 17000 + 1 * 87000, SDS-PAGE | Cupriavidus necator |
18924 | - |
1 * 93309 + 1 * 18924, calculated from sequence | Cupriavidus necator |
87000 | - |
1 * 17000 + 1 * 87000, SDS-PAGE | Cupriavidus necator |
92000 | - |
gel filtration | Cupriavidus necator |
93309 | - |
1 * 93309 + 1 * 18924, calculated from sequence | Cupriavidus necator |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Cupriavidus necator | an insertion in the napA gene leads to a complete loss of enzyme activity but does not abolish the ability of Alcaligenes eutrophus to use nitrate as a nitrogen source or as an electron acceptor in anaerobic respiration. Nevertheless, the NAP-deficient mutant shows delayed growth after transition from aerobic to anaerobic respiration, suggesting a role for periplasmic nitrate reductase in the adaptation to anaerobic metabolism | ? | - |
? | |
additional information | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | an insertion in the napA gene leads to a complete loss of enzyme activity but does not abolish the ability of Alcaligenes eutrophus to use nitrate as a nitrogen source or as an electron acceptor in anaerobic respiration. Nevertheless, the NAP-deficient mutant shows delayed growth after transition from aerobic to anaerobic respiration, suggesting a role for periplasmic nitrate reductase in the adaptation to anaerobic metabolism | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | - |
- |
- |
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Cupriavidus necator |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | an insertion in the napA gene leads to a complete loss of enzyme activity but does not abolish the ability of Alcaligenes eutrophus to use nitrate as a nitrogen source or as an electron acceptor in anaerobic respiration. Nevertheless, the NAP-deficient mutant shows delayed growth after transition from aerobic to anaerobic respiration, suggesting a role for periplasmic nitrate reductase in the adaptation to anaerobic metabolism | Cupriavidus necator | ? | - |
? | |
additional information | an insertion in the napA gene leads to a complete loss of enzyme activity but does not abolish the ability of Alcaligenes eutrophus to use nitrate as a nitrogen source or as an electron acceptor in anaerobic respiration. Nevertheless, the NAP-deficient mutant shows delayed growth after transition from aerobic to anaerobic respiration, suggesting a role for periplasmic nitrate reductase in the adaptation to anaerobic metabolism | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | ? | - |
? | |
nitrate + reduced benzyl viologen | - |
Cupriavidus necator | nitrite + oxidized benzyl viologen + H2O | - |
? | |
nitrate + reduced benzyl viologen | - |
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | nitrite + oxidized benzyl viologen + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 17000 + 1 * 87000, SDS-PAGE | Cupriavidus necator |
heterodimer | 1 * 93309 + 1 * 18924, calculated from sequence | Cupriavidus necator |
Synonyms | Comment | Organism |
---|---|---|
NAP | - |
Cupriavidus necator |
nitrate reductase, periplasmic | - |
Cupriavidus necator |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Cupriavidus necator |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
assay at | Cupriavidus necator |