Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli C41(DE3) cells | Chlamydia trachomatis |
gene dsbB, recombinant expression of recombinant enzyme in Escherichia coli, coexpression with Chlamydia trachomatis dsbA | Chlamydia trachomatis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of mutant CtDsbB-CCSS, in which periplasmic loop 2 Cys98 and Cys104 are mutated to serines. In the presence of CtDsbB-CCSS or CtDsbB-SSCC, CtDsbA catalysed oxidation of the peptide substrate is markedly reduced relative to wild-type CtDsbB, although oxidation proceeds more rapidly than observed for negative controls containing only buffer, or the wild-type CtDsbB variant alone. The disulfide bonds present in periplasmic loops P1 and P2 of CtDsbB are each required for complete oxidation of CtDsbA | Chlamydia trachomatis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Chlamydia trachomatis | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[DsbA protein] with reduced L-cysteine residues + quinone | Chlamydia trachomatis | overall reaction | [DsbA protein] carrying a disulfide bond + quinol | - |
? | |
[DsbA protein] with reduced L-cysteine residues + quinone | Chlamydia trachomatis A2497 | overall reaction | [DsbA protein] carrying a disulfide bond + quinol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydia trachomatis | - |
- |
- |
Chlamydia trachomatis | G4NNC5 | serovar A | - |
Chlamydia trachomatis A2497 | G4NNC5 | serovar A | - |
Purification (Comment) | Organism |
---|---|
His-Trap column chromatography and Superdex 200 gel filtration | Chlamydia trachomatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | membranes containing CtDsbB can sustain CtDsbA catalysed oxidation of a model substrate. Detergent solubilised, purified CtDsbB partially oxidises reduced CtDsbA, the active site CSAC thiols are the likely target for CtDsbB mediated oxidation | Chlamydia trachomatis | ? | - |
- |
|
additional information | membranes containing CtDsbB can sustain CtDsbA catalysed oxidation of a model substrate. Detergent solubilised, purified CtDsbB partially oxidises reduced CtDsbA, the active site CSAC thiols are the likely target for CtDsbB mediated oxidation | Chlamydia trachomatis A2497 | ? | - |
- |
|
[DsbA protein] with reduced L-cysteine residues + quinone | overall reaction | Chlamydia trachomatis | [DsbA protein] carrying a disulfide bond + quinol | - |
? | |
[DsbA protein] with reduced L-cysteine residues + quinone | overall reaction | Chlamydia trachomatis A2497 | [DsbA protein] carrying a disulfide bond + quinol | - |
? | |
[DsbA protein] with reduced L-cysteine residues + ubiquinone-1 | overall reaction | Chlamydia trachomatis | [DsbA protein] carrying a disulfide bond + ubiquinol-1 | - |
? | |
[DsbA protein] with reduced L-cysteine residues + ubiquinone-1 | overall reaction | Chlamydia trachomatis A2497 | [DsbA protein] carrying a disulfide bond + ubiquinol-1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
C. trachomatis disulfide bond protein B | - |
Chlamydia trachomatis |
CtDsbB | - |
Chlamydia trachomatis |
disulfide bond protein B | - |
Chlamydia trachomatis |
DsbB | - |
Chlamydia trachomatis |
Organism | Comment | Expression |
---|---|---|
Chlamydia trachomatis | CtDsbA protein is detected in Chlamydia trachomatis inclusion vacuoles at 20 h post infection, with more detected at 32 and similar levels at 44 h post infection as the developmental cycle proceeds | up |
General Information | Comment | Organism |
---|---|---|
physiological function | disulfide bond protein A (DsbA) is the primary oxidase in the disulfide oxidative pathway of bacteria. DsbA catalyses the introduction of disulfide bonds into reduced and folding proteins in concert with a membrane protein partner DsbB. DsbB uses a quinone cofactor as an electron acceptor, and together the DsbA-DsbB pair ultimately shuttle electrons from a reduced protein substrate to molecular oxygen via the respiratory pathway. CtDsbA is directly oxidised by CtDsbB, in a reaction in which both periplasmic cysteine pairs of CtDsbB are required for complete activity. The second disulfide of CtDsbA does not influence interaction with CtDsbB | Chlamydia trachomatis |