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Literature summary for 1.8.4.16 extracted from

  • Gordon, E.; Page, M.; Willis, A.; Ferguson, S.
    Escherichia coli DipZ Anatomy of a transmembrane protein disulphide reductase in which three pairs of cysteine residues, one in each of three domains, contribute differentially to function (2000), Mol. Microbiol., 35, 1360-1374 .
    View publication on PubMed
Search for more literature for 1.8.4.16

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JCB606 cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
C103A/C109A Echerichia coli expressing the C103A and C109A mutations is Cu2+ sensitive and shows no enzymatic activity Escherichia coli
C163A/C282A Echerichia coli expressing the C163A and C282A mutations is Cu2+ resistant Escherichia coli
C163A/C285A Echerichia coli expressing the C163A and C285A mutations is Cu2+ sensitive and shows no enzymatic activity Escherichia coli
C282A/C285A Echerichia coli expressing the C282A and C285A mutations is Cu2+ resistant Escherichia coli
C461A/C464A Echerichia coli expressing the C461A and C464A mutations is Cu2+ sensitive Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasmic membrane
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 Escherichia coli
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-

Organism

Organism UniProt Comment Textmining
Escherichia coli P36655
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-

Purification (Commentary)

Purification (Comment) Organism
Ni-Sepharose column chromatography, HiTrap chelating column chromatography, and DEAE-Sepharose column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme transfers reducing power from the cytoplasm to the periplasm so as to facilitate the formation of correct disulphide bonds and c-type cytochromes in the latter compartment Escherichia coli ?
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-

Synonyms

Synonyms Comment Organism
dipZ
-
 Escherichia coli