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Literature summary for 1.8.4.16 extracted from

  • Mavridou, D.; Stevens, J.; Goddard, A.; Willis, A.; Ferguson, S.; Redfield, C.
    Control of periplasmic interdomain thiol disulfide exchange in the transmembrane oxidoreductase DsbD (2009), J. Biol. Chem., 284, 3219-3226 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.4.16

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
C103A active site mutant Escherichia coli
C464A active site mutant Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasmic membrane
-
 Escherichia coli
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a [protein] carrying a disulfide bond + thioredoxin Escherichia coli overall reaction a [protein] with reduced L-cysteine residues + thioredoxin disulfide ?
-

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Strep-Tactin Sepharose column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a [protein] carrying a disulfide bond + thioredoxin overall reaction Escherichia coli a [protein] with reduced L-cysteine residues + thioredoxin disulfide ?
-
additional information the enzyme includes three domains, each containing a pair of cysteine residues that perform a series of disulfide exchange reactions. In the first step, the transmembrane domain accepts electrons from thioredoxin in the cytoplasm; these are then transferred to the periplasmic C-terminal domain and finally to the N-terminal domain, which is also located in the periplasm Escherichia coli ?
-
-

Subunits

Subunits Comment Organism
? x * 49000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
DsbD
-
 Escherichia coli