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Literature summary for 1.8.3.2 extracted from

  • Kuang, W.; Zhang, H.; Wang, M.; Zhou, N.-Y.; Deng, F.; Wang, H.; Gong, P.; Hu, Z.
    Three conserved regions in baculovirus sulfhydryl oxidase P33 are critical for enzymatic activity and function (2017), J. Virol., 91, e01158-17 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.3.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain BL21(DE3) Autographa californica nucleopolyhedrovirus

Crystallization (Commentary)

Crystallization (Comment) Organism
vapor diffusion sitting drops method at 16°C Autographa californica nucleopolyhedrovirus

Protein Variants

Protein Variants Comment Organism
E174A dimer interface mutant. The kcat-value of the mutant enzyme is 3.1fold lower than the value of the wild-type enyme Autographa californica nucleopolyhedrovirus
E174A/H227A dimer interface mutant. The kcat-value of the mutant enzyme is 6.8fold lower than the value of the wild-type enyme Autographa californica nucleopolyhedrovirus
E174K dimer interface mutant. The kcat-value of the mutant enzyme is 6.7fold lower than the value of the wild-type enyme Autographa californica nucleopolyhedrovirus
E183D salt bridge mutant. The kcat-value of the mutant enzyme is 1.2fold lower than the value of the wild-type enyme Autographa californica nucleopolyhedrovirus
E183R salt bridge mutant. The kcat-value of the mutant enzyme is 1.1fold higher than the value of the wild-type enyme Autographa californica nucleopolyhedrovirus
H114A active-site mutant. The occlusion bodies (OBs) of the mutant have a ragged surface and contain mostly occlusion-derived virus with a single nucleocapsid (ODVs). The occlusion bodies (OBs) of the mutant contain lower numbers of ODVs and have a significantly reduced oral infectivity in comparison to control virus. The kcat-value of the mutant enzyme is 4.1fold lower than the value of the wild-type enyme Autographa californica nucleopolyhedrovirus
H161A active site mutant. kcat value is too low to be reliably quantitated Autographa californica nucleopolyhedrovirus
H227A dimer interface mutant. The kcat-value of the mutant enzyme is 3.4fold lower than the value of the wild-type enyme Autographa californica nucleopolyhedrovirus
H227D dimer interface mutant. The occlusion bodies (OBs) of the mutant contain lower numbers of ODVs and have a significantly reduced oral infectivity in comparison to control virus. kcat value is too low to be reliably quantitated Autographa californica nucleopolyhedrovirus
Q235A active site mutant. kcat value is too low to be reliably quantitated Autographa californica nucleopolyhedrovirus
R127A/E183A salt bridge mutant. The occlusion bodies (OBs) of the mutant have a ragged surface and contain mostly occlusion-derived virus with a single nucleocapsid (ODVs). The occlusion bodies (OBs) of the mutant contain lower numbers of ODVs and have a significantly reduced oral infectivity in comparison to control virus. The kcat-value of the mutant enzyme is 1.2fold lower than the value of the wild-type enyme Autographa californica nucleopolyhedrovirus
R127E salt bridge mutant. The kcat-value of the mutant enzyme is 1.4fold lower than the value of the wild-type enyme Autographa californica nucleopolyhedrovirus
R127E/E183R salt bridge mutant. The kcat-value of the mutant enzyme is 1.1fold lower than the value of the wild-type enyme Autographa californica nucleopolyhedrovirus

Organism

Organism UniProt Comment Textmining
Autographa californica nucleopolyhedrovirus P41480
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,5'-dithiobis(2-nitrobenzoic acid) + O2
-
 Autographa californica nucleopolyhedrovirus ? + H2O
-
 ?

Synonyms

Synonyms Comment Organism
AcMNPV P33
-
 Autographa californica nucleopolyhedrovirus
sulfhydryl oxidase, P33
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 Autographa californica nucleopolyhedrovirus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.188
-
 5,5'-dithiobis(2-nitrobenzoic acid) pH 7.4, 37°C, dimer interface mutant E174A/H227A Autographa californica nucleopolyhedrovirus
0.19
-
 5,5'-dithiobis(2-nitrobenzoic acid) pH 7.4, 37°C, dimer interface mutant E174K Autographa californica nucleopolyhedrovirus
0.31
-
 5,5'-dithiobis(2-nitrobenzoic acid) pH 7.4, 37°C, active site mutant H161A Autographa californica nucleopolyhedrovirus
0.37
-
 5,5'-dithiobis(2-nitrobenzoic acid) pH 7.4, 37°C, dimer interface mutant H227A Autographa californica nucleopolyhedrovirus
0.411
-
 5,5'-dithiobis(2-nitrobenzoic acid) pH 7.4, 37°C, dimer interface mutant E174A Autographa californica nucleopolyhedrovirus
0.9
-
 5,5'-dithiobis(2-nitrobenzoic acid) pH 7.4, 37°C, salt bridge mutant R127E Autographa californica nucleopolyhedrovirus
1.048
-
 5,5'-dithiobis(2-nitrobenzoic acid) pH 7.4, 37°C, salt bridge mutant R127A/E183A Autographa californica nucleopolyhedrovirus
1.165
-
 5,5'-dithiobis(2-nitrobenzoic acid) pH 7.4, 37°C, salt bridge mutant E183R Autographa californica nucleopolyhedrovirus
1.19
-
 5,5'-dithiobis(2-nitrobenzoic acid) pH 7.4, 37°C, salt bridge mutant R127E/E183R Autographa californica nucleopolyhedrovirus
1.275
-
 5,5'-dithiobis(2-nitrobenzoic acid) pH 7.4, 37°C, wild-type enzyme Autographa californica nucleopolyhedrovirus
1.36
-
 5,5'-dithiobis(2-nitrobenzoic acid) pH 7.4, 37°C, salt bridge mutant E183D Autographa californica nucleopolyhedrovirus

General Information

General Information Comment Organism
physiological function sulfhydryl oxidase P33 is necessary for budded virus (BV) production and multinucleocapsid occlusion-derived virus (ODV) formation Autographa californica nucleopolyhedrovirus