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Literature summary for 1.8.1.4 extracted from

  • Dai, F.; Zhang, W.; Zhuang, Q.; Shao, Y.; Zhao, X.; Lv, Z.; Li, C.
    Dihydrolipoamide dehydrogenase of Vibrio splendidus is involved in adhesion to Apostichopus japonicus (2019), Virulence, 10, 839-848 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Vibrio splendidus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane outer membrane Vibrio splendidus 16020
-

Organism

Organism UniProt Comment Textmining
Vibrio splendidus
-
-
-
Vibrio splendidus LGP32
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA Sepharose column chromatography Vibrio splendidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-lipoamide + NADH + H+
-
 Vibrio splendidus DL-dihydrolipoamide + NAD+
-
 ?
DL-lipoamide + NADH + H+
-
 Vibrio splendidus LGP32 DL-dihydrolipoamide + NAD+
-
 ?

Subunits

Subunits Comment Organism
? x * 50900, isoform DLD1, calculated from amino acid sequence Vibrio splendidus
? x * 51000, isoform DLD1, SDS-PAGE Vibrio splendidus
? x * 53000, isoform DLD2, SDS-PAGE Vibrio splendidus
? x * 53160, isoform DLD2, calculated from amino acid sequence Vibrio splendidus

Synonyms

Synonyms Comment Organism
dihydrolipoamide dehydrogenase
-
 Vibrio splendidus
DLD1 isoform Vibrio splendidus
Dld2 isoform Vibrio splendidus

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Vibrio splendidus

pI Value

Organism Comment pI Value Maximum pI Value
Vibrio splendidus isoform DLD2, calculated from amino acid sequence
-
 5.3
Vibrio splendidus isoform DLD1, calculated from amino acid sequence
-
 5.6

General Information

General Information Comment Organism
physiological function the enzyme is involved in adhesion to polystyrene as well as coelomocytes and other tissues like body wall, tentacle, muscle, respiratory tree and intestine from sea cucumber Apostichopus japonicas Vibrio splendidus