Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
P355A | the mutation makes enzyme binding to NAD+ substantially less efficient. The catalytic efficiency of the mutant toward NAD+ is decreased by 81% compared to the wild type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
NAD+ | wild type enzyme, at pH 8.0 and 37°C | Homo sapiens | |
0.52 | - |
dihydrolipoamide | mutant enzyme P355A, at pH 8.0 and 37°C | Homo sapiens | |
0.64 | - |
dihydrolipoamide | wild type enzyme, at pH 8.0 and 37°C | Homo sapiens | |
0.7 | - |
NAD+ | mutant enzyme P355A, at pH 8.0 and 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P09622 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel affinity column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydrolipoamide + NAD+ | - |
Homo sapiens | lipoamide + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 50000, SDS-PAGE | Homo sapiens |
homodimer | 2 * 50216, calculated from amino acid sequence | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
dihydrolipoamide dehydrogenase | - |
Homo sapiens |
dihydrolipoamide:NAD+ oxidoreductase | - |
Homo sapiens |
E3 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
624 | - |
NAD+ | mutant enzyme P355A, at pH 8.0 and 37°C | Homo sapiens | |
624 | - |
dihydrolipoamide | mutant enzyme P355A, at pH 8.0 and 37°C | Homo sapiens | |
899 | - |
NAD+ | wild type enzyme, at pH 8.0 and 37°C | Homo sapiens | |
899 | - |
dihydrolipoamide | wild type enzyme, at pH 8.0 and 37°C | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Homo sapiens | |
NAD+ | - |
Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
891 | - |
NAD+ | mutant enzyme P355A, at pH 8.0 and 37°C | Homo sapiens | |
1200 | - |
dihydrolipoamide | mutant enzyme P355A, at pH 8.0 and 37°C | Homo sapiens | |
1405 | - |
dihydrolipoamide | wild type enzyme, at pH 8.0 and 37°C | Homo sapiens | |
4732 | - |
NAD+ | wild type enzyme, at pH 8.0 and 37°C | Homo sapiens |