D413A |
substitutions has no large effects on E3 activity when measured in its free form. However, when reconstituted in the complex, the pyruvate dehydrogenase activity is reduced to 18%. The binding affinities of the mutant to the di-domain of the E3-binding protein are severely reduced |
Homo sapiens |
Y438A |
substitutions has no large effects on E3 activity when measured in its free form. However, when reconstituted in the complex, the pyruvate dehydrogenase activity is reduced to 9%. The binding affinities of the mutant to the di-domain of the E3-binding protein are severely reduced and binding of is accompanied by an unfavorable enthalpy change and a large positive entropy change |
Homo sapiens |
Y438H |
substitutions has no large effects on E3 activity when measured in its free form. However, when reconstituted in the complex, the pyruvate dehydrogenase activity is reduced to 20%. The binding affinities of the mutant to the di-domain of the E3-binding protein ire severely reduced |
Homo sapiens |