Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hydroxylamine | potent inhibitor | Methylacidiphilum fumariolicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0014 | - |
hydroxylamine | with bovine cytochrome c as electron acceptor, at pH 7.5 and 60°C | Methylacidiphilum fumariolicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylacidiphilum fumariolicum | - |
- |
- |
Methylacidiphilum fumariolicum SolV | - |
- |
- |
Purification (Comment) | Organism |
---|---|
SP Sepharose column chromatography and Superdex 200 gel filtration | Methylacidiphilum fumariolicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydroxylamine + 3 bovine ferricytochrome c | - |
Methylacidiphilum fumariolicum | nitric oxide + 3 bovine ferrocytochrome c + 3 H+ | - |
? | |
hydroxylamine + 3 bovine ferricytochrome c | - |
Methylacidiphilum fumariolicum SolV | nitric oxide + 3 bovine ferrocytochrome c + 3 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 83860, calculated from amino acid sequence | Methylacidiphilum fumariolicum |
Synonyms | Comment | Organism |
---|---|---|
hydroxylamine oxidoreductase | - |
Methylacidiphilum fumariolicum |
mHAO | - |
Methylacidiphilum fumariolicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
the enzyme maintains about 80% of its maximum activity at temperatures above 60°C | Methylacidiphilum fumariolicum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.2 | - |
hydroxylamine | with bovine cytochrome c as electron acceptor, at pH 7.5 and 60°C | Methylacidiphilum fumariolicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme c | - |
Methylacidiphilum fumariolicum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00052 | - |
hydroxylamine | at pH 7.5 and 60°C | Methylacidiphilum fumariolicum |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme allows methanotrophs to thrive under high ammonia concentrations in natural and engineered ecosystems, such as those observed in rice paddy fields, landfills, or volcanic mud pots, by preventing the accumulation of inhibitory hydroxylamine | Methylacidiphilum fumariolicum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5100 | - |
hydroxylamine | with bovine cytochrome c as electron acceptor, at pH 7.5 and 60°C | Methylacidiphilum fumariolicum |