Protein Variants | Comment | Organism |
---|---|---|
D439N | about 7% of wild-type activity | Bradyrhizobium sp. ORS 375 |
additional information | construction of domain-truncated mutants lacking the Cyt c (18% of wild-type activity) and the Cyt c-Cup domains (3fold increase in activity) | Bradyrhizobium sp. ORS 375 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
and 92000, gel filtration of mutant lacking the cyt c and cup domain | Bradyrhizobium sp. ORS 375 |
92000 | - |
and 40000, gel filtration of mutant lacking the cyt c and cup domain | Bradyrhizobium sp. ORS 375 |
114000 | - |
and 260000, gel filtration of mutant lacking the cyt c domain | Bradyrhizobium sp. ORS 375 |
192000 | - |
and 391000, gel filtration of wild-type | Bradyrhizobium sp. ORS 375 |
260000 | - |
and 114000, gel filtration of mutantlacking the cyt c domain | Bradyrhizobium sp. ORS 375 |
391000 | - |
and 192000, gel filtration of wild-type | Bradyrhizobium sp. ORS 375 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bradyrhizobium sp. ORS 375 | H0SHH5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrite + reduced phenazine methosulfate | - |
Bradyrhizobium sp. ORS 375 | NO + oxidized phenazine methosulfate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BRAO375_2740002 | - |
Bradyrhizobium sp. ORS 375 |
General Information | Comment | Organism |
---|---|---|
metabolism | 4-domain variant of enzyme utilizes N-terminal tethering for downregulating enzymatic activity. Tethering communicates the redox state of the heme to the distant type 2 copper center that helps initiate substrate binding for catalysis | Bradyrhizobium sp. ORS 375 |
physiological function | strain has two copies of nirK, a 4-domain NiR. and a 2-domain NiR, referred to for clarity as 2DNiR. 2dNir shows anbout 5% of the activtiy of the 4-domain variant | Bradyrhizobium sp. ORS 375 |