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Literature summary for 1.7.1.3 extracted from

  • Chu, S.; Zhang, D.; Wang, D.; Zhi, Y.; Zhou, P.
    Heterologous expression and biochemical characterization of assimilatory nitrate and nitrite reductase reveals adaption and potential of Bacillus megaterium NCT-2 in secondary salinization soil (2017), Int. J. Biol. Macromol., 101, 1019-1028 .
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Priestia megaterium

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM, 76% of initial activity Priestia megaterium
additional information not inhibitory nor activating: Ba2+, Zn2+, EDTA Priestia megaterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.67
-
 nitrate pH 7.1, 30°C Priestia megaterium

Metals/Ions

Metals/Ions Comment Organism Structure
Al3+ 1 mM, 113% of initial activity Priestia megaterium
Ca2+ 1 mM, 136% of initial activity Priestia megaterium
Fe2+ 1 mM, 137% of initial activity Priestia megaterium
Mg2+ 1 mM, 120% of initial activity Priestia megaterium
Mn2+ 1 mM, 114% of initial activity Priestia megaterium
additional information not inhibitory nor activating: Ba2+, Zn2+, EDTA Priestia megaterium

Organism

Organism UniProt Comment Textmining
Priestia megaterium A0A8D3X5C3 and A0A8D3X5J3 A0A8D3X5C3 i.e catalytic subunit NasC, A0A8D3X5J3 i.e. electron transfer subunit NasD
-
Priestia megaterium WSH-002 A0A8D3X5C3 and A0A8D3X5J3 A0A8D3X5C3 i.e catalytic subunit NasC, A0A8D3X5J3 i.e. electron transfer subunit NasD
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD(P)H + H+ + nitrate
-
 Priestia megaterium NAD(P)+ + nitrite + H2O
-
 ?
NAD(P)H + H+ + nitrate
-
 Priestia megaterium WSH-002 NAD(P)+ + nitrite + H2O
-
 ?
reduced methyl viologen + nitrate the optimum electron donor is methyl viologen plus Na2S2O4 Priestia megaterium oxidized methyl viologen + nitrite + H2O
-
 ?
reduced methyl viologen + nitrate the optimum electron donor is methyl viologen plus Na2S2O4 Priestia megaterium WSH-002 oxidized methyl viologen + nitrite + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 87300, electron transfer subunit, x * 80500 catalytic subunit, SDS-PAGE Priestia megaterium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Priestia megaterium

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40
-
 more than 90% of maximum activity Priestia megaterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
-
 Priestia megaterium

pH Range

pH Minimum pH Maximum Comment Organism
6.5
-
 more than 99% of maximum activity Priestia megaterium