Literature summary for 1.7.1.13 extracted from
Jung, J.; Braun, J.; Czabany, T.; Nidetzky, B.
Interplay of nucleophilic catalysis with proton transfer in the nitrile reductase QueF from Escherichia coli (2019), Catal. Sci. Technol., 9, 842-853 .
No PubMed abstract available
Cloned(Commentary)
Cloned (Comment) |
Organism |
expressed in Escherichia coli BL21(DE3) cells |
Escherichia coli |
Protein Variants
Protein Variants |
Comment |
Organism |
C190A |
the mutation annihilates preQ0 covalent binding and largely disrupts the nitrile-to-amine reductase activity |
Escherichia coli |
C190S |
the mutation annihilates preQ0 covalent binding and largely disrupts the nitrile-to-amine reductase activity |
Escherichia coli |
D197A |
the variant does not take up protons in conjunction with preQ0 binding |
Escherichia coli |
D197H |
the variant recovers proton uptake to a level almost analogous to that seen with the wildtype enzyme |
Escherichia coli |
H229A |
the variant readily forms the thioimidate adduct and is 24fold less active for preQ0 reduction than wild type enzyme |
Escherichia coli |
Natural Substrates/ Products (Substrates)
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
7-cyano-7-deazaguanine + 2 NADPH + 2 H+ |
Escherichia coli |
i.e. preQ0 |
7-aminomethyl-7-deazaguanine + 2 NADP+ |
- |
? |
|
Organism
Organism |
UniProt |
Comment |
Textmining |
Escherichia coli |
Q46920 |
- |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
immobilized metal ion affinity column chromatography and gel filtration |
Escherichia coli |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
7-cyano-7-deazaguanine + 2 NADPH + 2 H+ |
i.e. preQ0 |
Escherichia coli |
7-aminomethyl-7-deazaguanine + 2 NADP+ |
- |
? |
|
Synonyms
Synonyms |
Comment |
Organism |
QueF |
- |
Escherichia coli |