Cloned (Comment) | Organism |
---|---|
native and truncated form expressed in Escherichia coli BL21(DE3)pLysS as His tag fusion protein | Phanerodontia chrysosporium |
Protein Variants | Comment | Organism |
---|---|---|
DELTA1-23 | truncated enzyme | Phanerodontia chrysosporium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0019 | - |
NADPH | full length enzyme, pH 7.8, 25°C | Phanerodontia chrysosporium | |
0.0022 | - |
NADPH | truncated enzyme, pH 7.8, 25°C | Phanerodontia chrysosporium | |
0.011 | - |
ferricytochrome c | full length enzyme, pH 7.8, 25°C | Phanerodontia chrysosporium | |
0.012 | - |
ferricytochrome c | truncated enzyme, pH 7.8, 25°C | Phanerodontia chrysosporium |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Phanerodontia chrysosporium | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
79300 | - |
theoretical mass, truncated enzyme | Phanerodontia chrysosporium |
81600 | - |
theoretical mass, full length enzyme | Phanerodontia chrysosporium |
85900 | - |
SDS-PAGE, truncated enzyme | Phanerodontia chrysosporium |
88200 | - |
SDS-PAGE, full length enzyme | Phanerodontia chrysosporium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricytochrome P450 + NADPH | Phanerodontia chrysosporium | transfer of electrons during the CYP catalytic cycle | 2 ferrocytochrome P450 + NADP+ + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phanerodontia chrysosporium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzymes using His-tag | Phanerodontia chrysosporium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.27 | - |
purified full length enzyme, pH 7.8, 25°C | Phanerodontia chrysosporium |
14.76 | - |
purified truncated enzyme, pH 7.8, 25°C | Phanerodontia chrysosporium |
Storage Stability | Organism |
---|---|
-80°C, 20 mM phosphate buffer, pH 7.5, 20% glycerol | Phanerodontia chrysosporium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricytochrome c + NADPH | - |
Phanerodontia chrysosporium | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
2 ferricytochrome P450 + NADPH | transfer of electrons during the CYP catalytic cycle | Phanerodontia chrysosporium | 2 ferrocytochrome P450 + NADP+ + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NADPH-cytochrome P450 reductase | - |
Phanerodontia chrysosporium |
NADPH-ferrihemoprotein reductase | - |
Phanerodontia chrysosporium |
NADPH:ferrihemoprotein oxidoreductase | - |
Phanerodontia chrysosporium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
19 | - |
ferricytochrome c | full length enzyme, pH 7.8, 25°C | Phanerodontia chrysosporium | |
42 | - |
ferricytochrome c | truncated enzyme, pH 7.8, 25°C | Phanerodontia chrysosporium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | together with FMN | Phanerodontia chrysosporium | |
FMN | together with FAD | Phanerodontia chrysosporium | |
NADPH | - |
Phanerodontia chrysosporium |