Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrobaculum islandicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
2,6-dichlorophenolindophenol | pH 8.0, 50°C | Pyrobaculum islandicum | |
4.2 | - |
D-proline | pH 8.0, 50°C | Pyrobaculum islandicum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | associated with | Pyrobaculum islandicum | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40341 | - |
4 * 40341, calculated from sequence | Pyrobaculum islandicum |
42000 | - |
4 * 42000, SDS-PAGE | Pyrobaculum islandicum |
145000 | - |
gel filtration | Pyrobaculum islandicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrobaculum islandicum | A1RVM8 | JCM 9189 | - |
Pyrobaculum islandicum JCM 9189 | A1RVM8 | JCM 9189 | - |
Purification (Comment) | Organism |
---|---|
- |
Pyrobaculum islandicum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.3 | - |
enzyme from Pyrobaculum islandicum | Pyrobaculum islandicum |
13.2 | - |
recombinant enzyme | Pyrobaculum islandicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
allo-4-hydroxy-D-proline + oxidized 2,6-dichloroindophenol + H2O | 89% of the activity with D-proline | Pyrobaculum islandicum | ? + reduced 2,6-dichloroindophenol | - |
? | |
allo-4-hydroxy-D-proline + oxidized 2,6-dichloroindophenol + H2O | 89% of the activity with D-proline | Pyrobaculum islandicum JCM 9189 | ? + reduced 2,6-dichloroindophenol | - |
? | |
D-alanine + oxidized 2,6-dichloroindophenol + H2O | 26% of the activity with D-proline | Pyrobaculum islandicum | pyruvate + NH3 + reduced 2,6-dichloroindophenol | - |
? | |
D-alanine + oxidized 2,6-dichloroindophenol + H2O | 26% of the activity with D-proline | Pyrobaculum islandicum JCM 9189 | pyruvate + NH3 + reduced 2,6-dichloroindophenol | - |
? | |
D-arginine + oxidized 2,6-dichloroindophenol + H2O | 10% of the activity with D-proline | Pyrobaculum islandicum | 2-oxo-5-guanidinopentanoate + NH3 + reduced 2,6-dichloroindophenol | - |
? | |
D-arginine + oxidized 2,6-dichloroindophenol + H2O | 10% of the activity with D-proline | Pyrobaculum islandicum JCM 9189 | 2-oxo-5-guanidinopentanoate + NH3 + reduced 2,6-dichloroindophenol | - |
? | |
D-aspartate + oxidized 2,6-dichloroindophenol + H2O | 21% of the activity with D-proline | Pyrobaculum islandicum | oxaloacetate + NH3 + reduced 2,6-dichloroindophenol | - |
? | |
D-aspartate + oxidized 2,6-dichloroindophenol + H2O | 21% of the activity with D-proline | Pyrobaculum islandicum JCM 9189 | oxaloacetate + NH3 + reduced 2,6-dichloroindophenol | - |
? | |
D-glutamate + oxidized 2,6-dichloroindophenol + H2O | 23% of the activity with D-proline | Pyrobaculum islandicum | 2-oxo-glutarate + NH3 + reduced 2,6-dichloroindophenol | - |
? | |
D-histidine + oxidized 2,6-dichloroindophenol + H2O | 30% of the activity with D-proline | Pyrobaculum islandicum | 3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + reduced 2,6-dichloroindophenol | - |
? | |
D-isoleucine + oxidized 2,6-dichloroindophenol + H2O | 49% of the activity with D-proline | Pyrobaculum islandicum | 3-methyl-2-oxopropanoate + NH3 + reduced 2,6-dichloroindophenol | - |
? | |
D-leucine + oxidized 2,6-dichloroindophenol + H2O | 39% of the activity with D-proline | Pyrobaculum islandicum | 4-methyl-2-oxopentanoate + NH3 + reduced 2,6-dichloroindophenol | - |
? | |
D-phenylalanine + oxidized 2,6-dichloroindophenol + H2O | 28% of the activity with D-proline | Pyrobaculum islandicum | phenylpyruvate + NH3 + reduced 2,6-dichloroindophenol | - |
? | |
D-proline + 2,6-dichlorophenolindophenol | - |
Pyrobaculum islandicum | 1-pyrroline-2-carboxylate + reduced 2,6-dichlorophenolindophenol | - |
r | |
D-proline + oxidized 2,6-dichloroindophenol | D-proline is the most preferred substrate among the D-amino acids. 2,6-Dichloroindophenol is the most preferred electron acceptor | Pyrobaculum islandicum | DELTA1-pyrroline 2-carboxylate + reduced 2,6-dichloroindophenol | - |
? | |
D-proline + oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide | 33% of the activity with oxidized 2,6-dichloroindophenol | Pyrobaculum islandicum | DELTA1-pyrroline 2-carboxylate + reduced 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide | - |
? | |
D-proline + oxidized phenazine methosulfate | 43% of the activity with oxidized 2,6-dichloroindophenol | Pyrobaculum islandicum | DELTA1-pyrroline 2-carboxylate + reduced phenazine methosulfate | - |
? | |
D-serine + oxidized 2,6-dichloroindophenol + H2O | 13% of the activity with D-proline | Pyrobaculum islandicum | 3-hydroxy-2-oxopropanoate + NH3 + reduced 2,6-dichloroindophenol | - |
? | |
D-threonine + oxidized 2,6-dichloroindophenol + H2O | 15% of the activity with D-proline | Pyrobaculum islandicum | 3-hydroxy-2-oxobutanoate + NH3 + reduced 2,6-dichloroindophenol | - |
? | |
D-tryptophan + oxidized 2,6-dichloroindophenol + H2O | 14% of the activity with D-proline | Pyrobaculum islandicum | 3-indole-2-oxopropanoate + NH3 + reduced 2,6-dichloroindophenol | - |
? | |
D-valine + oxidized 2,6-dichloroindophenol + H2O | 41% of the activity with D-proline | Pyrobaculum islandicum | 2-oxoisopentanoate + NH3 + reduced 2,6-dichloroindophenol | - |
? | |
additional information | ferricyanide, NAD+, NADP+, O2, menadione (vitamin K3), benzyl viologen, horse heart cytochrome c, and methylene blue are inert as electron acceptors | Pyrobaculum islandicum | ? | - |
? | |
additional information | ferricyanide, NAD+, NADP+, O2, menadione (vitamin K3), benzyl viologen, horse heart cytochrome c, and methylene blue are inert as electron acceptors | Pyrobaculum islandicum JCM 9189 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 42000, SDS-PAGE | Pyrobaculum islandicum |
tetramer | 4 * 40341, calculated from sequence | Pyrobaculum islandicum |
Synonyms | Comment | Organism |
---|---|---|
dye-linked D-proline dehydrogenase | - |
Pyrobaculum islandicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
the activity is linearly increased with increasing temperature to 70°C. The highest activity is above 70°C, and the correct assay is not achieved above 70°C, because of the non-enzymatic decolorization of 2,6-dichloroindophenol | Pyrobaculum islandicum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
10 min, enzyme retains its full activity | Pyrobaculum islandicum |
100 | - |
10 min, 90% loss of activity | Pyrobaculum islandicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
D-proline dehydrogenation | Pyrobaculum islandicum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | 50% of maximal activity at pH 6.5 and at pH 8.5, proline dehydrogenation | Pyrobaculum islandicum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | 50°C, 10 min, enzyme retains its full activity | Pyrobaculum islandicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | prosthetic group | Pyrobaculum islandicum |