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Literature summary for 1.5.1.5 extracted from

  • Haque, M.R.; Higashiura, A.; Nakagawa, A.; Hirowatari, A.; Furuya, S.; Yamamoto, K.
    Molecular structure of a 5,10-methylenetetrahydrofolate dehydrogenase from the silkworm Bombyx mori (2019), FEBS open bio, 9, 618-628 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.5.1.5

Cloned(Commentary)

Cloned (Comment) Organism
bmMTHFD, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli Bombyx mori

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant bmMTFDH in complex with, X-ray diffraction structure determination and analysis at 1.75 A resolution Bombyx mori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + NAD+ Bombyx mori
-
 5,10-methenyltetrahydrofolate + NADH + H+
-
 ?
5,10-methylenetetrahydrofolate + NADP+ Bombyx mori
-
 5,10-methenyltetrahydrofolate + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Bombyx mori
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + NAD+
-
 Bombyx mori 5,10-methenyltetrahydrofolate + NADH + H+
-
 ?
5,10-methylenetetrahydrofolate + NADP+
-
 Bombyx mori 5,10-methenyltetrahydrofolate + NADPH + H+
-
 ?
additional information the recombinant Bombyx mori MTHFD (bmMTHFD) recognizes 5,10-methylenetetrahydrofolate and 5,10-methenyltetrahydrofolate as substrates in the presence of NADP+ as well as NAD+ Bombyx mori ?
-
-

Synonyms

Synonyms Comment Organism
5,10-methylenetetrahydrofolate dehydrogenase
-
 Bombyx mori
bmMTHFD
-
 Bombyx mori
MTHFD
-
 Bombyx mori

Cofactor

Cofactor Comment Organism Structure
additional information the recombinant Bombyx mori MTHFD (bmMTHFD) recognizes 5,10-methylenetetrahydrofolate and 5,10-methenyltetrahydrofolate as substrates in the presence of NADP+ as well as NAD+ Bombyx mori
NAD+
-
 Bombyx mori
NADH
-
 Bombyx mori
NADP+
-
 Bombyx mori
NADPH
-
 Bombyx mori

General Information

General Information Comment Organism
additional information the amino acid residue Tyr49 contributes to its catalytic activity Bombyx mori