Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | 0.1 mM, 60% inhibition | Homo sapiens | |
CoCl2 | 0.01 mM, 30% inhibition | Homo sapiens | |
HgCl2 | 0.0001 mM, 60% inhibition | Homo sapiens | |
iodoacetamide | - |
Homo sapiens | |
additional information | not inhibited by EDTA, o-phenanthroline, and 2,2'-bipyridyl | Homo sapiens | |
N-ethylmaleimide | 1 mM, 76% inhibition | Homo sapiens | |
p-chloromercuribenzoate | 0.01 mM, 70% inhibition | Homo sapiens | |
tetrahydrobiopterin | above 0.05 mM, substrate inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
tetrahydrobiopterin | - |
Homo sapiens | |
0.029 | - |
NADH | - |
Homo sapiens | |
0.036 | - |
2-amino-4-hydroxy-6,7-dimethyl-5,6,7,8-tetrahydropteridine | - |
Homo sapiens | |
0.77 | - |
NADPH | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
2 * 26000, SDS-PAGE | Homo sapiens |
47500 | - |
- |
Homo sapiens |
50000 | - |
- |
Homo sapiens |
54000 | - |
gradient PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
quinonoid 7,8-(6H)-dihydrobiopterin + NAD(P)H | Homo sapiens | essential enzyme component of the complex system catalyzing hydroxylation of phenylalanine, tyrosine and tryptophan | 5,6,7,8-tetrahydrobiopterin + NAD(P)+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
422 | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-amino-4-hydroxy-6,7-dimethyl-quinonoid-dihydropteridine + NAD(P)H | NADH is 20fold more effective than NADPH | Homo sapiens | 2-amino-4-hydroxy-6,7-dimethyltetrahydropteridine + NAD(P)+ | - |
? | |
6,7-dihydropteridine + NADH | - |
Homo sapiens | (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin + NAD+ | - |
? | |
quinoid dihydrobiopterin + NAD(P)H | NADH is 2.5fold more effective than NADPH | Homo sapiens | tetrahydrobiopterin + NAD(P)+ | - |
? | |
quinonoid 7,8-(6H)-dihydrobiopterin + NAD(P)H | essential enzyme component of the complex system catalyzing hydroxylation of phenylalanine, tyrosine and tryptophan | Homo sapiens | 5,6,7,8-tetrahydrobiopterin + NAD(P)+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 26000, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
200 | - |
tetrahydrobiopterin | - |
Homo sapiens | |
510 | - |
2-amino-4-hydroxy-6,7-dimethyl-5,6,7,8-tetrahydropteridine | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | utilizes both NADH and NADPH | Homo sapiens | |
NADH | enzyme prefers NADH | Homo sapiens | |
NADPH | utilizes both NADH and NADPH | Homo sapiens | |
NADPH | NADH is preferred | Homo sapiens |