Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 2 mM, 45% inhibition, complete inhibition after preincubation with 1 mM EDTA for 30 min, almost the total activity can be restored with 2 mM Co2+, Ni2+ and Mn2+ | Rauvolfia serpentina | |
p-hydroxymercuribenzoate | 0.2 mM, complete inhibition | Rauvolfia serpentina |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | most probably a metalloprotein, complete inhibition after preincubation with 1 mM EDTA for 30 min, can be restored with 2 mM Co2+, Ni2+ and Mn2+ | Rauvolfia serpentina |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
gel filtration | Rauvolfia serpentina |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
vomilenine + NADPH | Rauvolfia serpentina | enzyme catalyzes a late step in the biosynthesis of the monoterpenoid indole alkaloid ajmaline | 2-beta-(R)-1,2-dihydrovomilenine + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rauvolfia serpentina | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, Source 30Q, Source 15Phe, 2',5'-ADP-Sepharose | Rauvolfia serpentina |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Rauvolfia serpentina | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0031 | - |
- |
Rauvolfia serpentina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
vomilenine + NADPH | - |
Rauvolfia serpentina | 2-beta-(R)-1,2-dihydrovomilenine + NADP+ | non-natural 2alpha-(S)-1,2-dihydrovomilenine is never observed | ? | |
vomilenine + NADPH | enzyme catalyzes a late step in the biosynthesis of the monoterpenoid indole alkaloid ajmaline | Rauvolfia serpentina | 2-beta-(R)-1,2-dihydrovomilenine + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 43000, SDS-PAGE | Rauvolfia serpentina |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
50% activity at 45°C | Rauvolfia serpentina |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
in phosphate buffer, 50% activity at pH 5.2 and pH 6.8 respectively | Rauvolfia serpentina |
6.5 | - |
in phosphate/citric acid buffer | Rauvolfia serpentina |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.2 | 6.8 | - |
Rauvolfia serpentina |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Rauvolfia serpentina |