Application | Comment | Organism |
---|---|---|
medicine | LAAO is involved in dermonecrosis in mice and shows cytotoxic effects in normal human keratinocytes, the major cell type in the epidermis, which undergoes extensive necrosis at the snakebite site. Pharmacological inhibition by N-acetyl cysteine prevents Bothrops atrox venom-induced necrosis. Treatment with purified LAAO decreases keratinocyte viability with an effective Concentration (EC50) of 5.1 microg/ml. Cytotoxicity caused by LAAO is mediated by H2O2 and treated cells undergo autophagy, followed by apoptosis and necrosis. LAAO induces morphological alterations that precede cell death | Bothrops atrox |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bothrops atrox | P0CC17 | - |
- |
Purification (Comment) | Organism |
---|---|
purification by molecular exclusion, ion exchange and affinity purification | Bothrops atrox |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Bothrops atrox | - |
Storage Stability | Organism |
---|---|
LAAO is more stable when kept at -80°C, followed by 4°C, reactivation using sodium acetate buffer pH 5.0 for 30 min at 37°C leads to increased activity | Bothrops atrox |
Subunits | Comment | Organism |
---|---|---|
? | x * 57000, SDS-PAGE and mass spectrometry | Bothrops atrox |