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Literature summary for 1.4.3.2 extracted from
- Insights into the structure, function and stability of bordonein-L, the first L-amino acid oxidase from Crotalus durissus terrificus snake venom (2019), Biochimie, 163, 33-49 .
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | the enzyme exhibits cytotoxicity against fibroblast cell line and kills Leishmania amazonensis promastigotes, intensified by substrate addition | Crotalus durissus terrificus |
General Stability
| General Stability | Organism |
|---|---|
| the enzyme tends to destabilize from neutral to basic pHs and in presence of mono/bivalent ions and it is highly stabilized by acid pHs and its substrates | Crotalus durissus terrificus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.14 | - |
L-phenylalanine | pH not specified in the publication, temperature not specified in the publication | Crotalus durissus terrificus |  |
| 0.48 | - |
L-tryptophan | pH not specified in the publication, temperature not specified in the publication | Crotalus durissus terrificus | |
| 0.58 | - |
L-leucine | pH not specified in the publication, temperature not specified in the publication | Crotalus durissus terrificus | |
| 0.97 | - |
L-methionine | pH not specified in the publication, temperature not specified in the publication | Crotalus durissus terrificus | |
| 3.9 | - |
L-isoleucine | pH not specified in the publication, temperature not specified in the publication | Crotalus durissus terrificus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Crotalus durissus terrificus | C0HJE7 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | sequence contains one N-glycosylation site and two disulfide bonds, protein shows about 5% carbohydrate content | Crotalus durissus terrificus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification by cationic exchange, size exclusion, and glycoprotein affinity | Crotalus durissus terrificus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| venom | - |
Crotalus durissus terrificus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.44 | - |
pH not specified in the publication, temperature not specified in the publication | Crotalus durissus terrificus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-isoleucine + H2O + O2 | - |
Crotalus durissus terrificus | 3-methyl-2-oxopentanoate + NH3 + H2O2 | - |
? | |
| L-leucine + H2O + O2 | - |
Crotalus durissus terrificus | 2-oxo-4-methylpentanoate + NH3 + H2O2 | - |
? | |
| L-methionine + H2O + O2 | - |
Crotalus durissus terrificus | 4-methylthio-2-oxobutanoate + NH3 + H2O2 | - |
? | |
| L-phenylalanine + O2 | - |
Crotalus durissus terrificus | phenylpyruvate + NH3 + H2O2 | - |
? | |
| L-tryptophan + H2O + O2 | - |
Crotalus durissus terrificus | indole-3-pyruvate + NH3 + H2O2 | - |
? | |
| additional information | enzyme shows highest enzymatic activities upon the following L-hydrophobic amino acids: Met, Leu, Trp, Phe and Ile. L-amino acids not oxidized by bordonein-L include Lys, Ser, Thr, Pro, Asp, Gly, Tyr, Cys and Glu. Low activity is observed with His and Arg. Gln, Val, Asn and Ala are even more poorly oxidized by bordonein-L | Crotalus durissus terrificus | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 113833, calculated from sequence | Crotalus durissus terrificus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| bordonein-L | - |
Crotalus durissus terrificus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3421 | - |
L-isoleucine | pH not specified in the publication, temperature not specified in the publication | Crotalus durissus terrificus | |
| 3483 | - |
L-phenylalanine | pH not specified in the publication, temperature not specified in the publication | Crotalus durissus terrificus | |
| 3720 | - |
L-tryptophan | pH not specified in the publication, temperature not specified in the publication | Crotalus durissus terrificus | |
| 5965 | - |
L-leucine | pH not specified in the publication, temperature not specified in the publication | Crotalus durissus terrificus | |
| 7284 | - |
L-methionine | pH not specified in the publication, temperature not specified in the publication | Crotalus durissus terrificus | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Crotalus durissus terrificus | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Crotalus durissus terrificus | calculated from sequence | - |
8.2 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 900 | - |
L-isoleucine | pH not specified in the publication, temperature not specified in the publication | Crotalus durissus terrificus | |
| 7500 | - |
L-methionine | pH not specified in the publication, temperature not specified in the publication | Crotalus durissus terrificus | |
| 7800 | - |
L-tryptophan | pH not specified in the publication, temperature not specified in the publication | Crotalus durissus terrificus | |
| 10000 | - |
L-leucine | pH not specified in the publication, temperature not specified in the publication | Crotalus durissus terrificus | |
| 25000 | - |
L-phenylalanine | pH not specified in the publication, temperature not specified in the publication | Crotalus durissus terrificus | |
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