Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Top10 cells | Microbacterium oxydans |
Protein Variants | Comment | Organism |
---|---|---|
L199F | the mutant is more active than the wild type enzyme toward the primary amines | Microbacterium oxydans |
L199I | the mutant is more active than the wild type enzyme toward the primary amines | Microbacterium oxydans |
L199T | the mutation enhances the enzyme activity toward the secondary amines | Microbacterium oxydans |
M226A | the mutant exhibits enhanced activity relative to the wild type enzyme for most (S)-enantiomers of primary amines and some secondary amines | Microbacterium oxydans |
M226I | the mutant is more active than the wild type enzyme toward the primary amines | Microbacterium oxydans |
M226T | the mutant is more active than the wild type enzyme toward the primary amines | Microbacterium oxydans |
T198A | the mutant exhibits enhanced activity relative to the wild type enzyme for most (S)-enantiomers of primary amines and some secondary amines | Microbacterium oxydans |
T198F | the mutation enhances the enzyme activity toward the secondary amines | Microbacterium oxydans |
T198F/L199S/M226F | the substrate 2-methyl-1, 2, 3, 4-tetrahydroquinoline is deracemized by the triple mutant | Microbacterium oxydans |
T198I | the mutant is more active than the wild type enzyme toward the primary amines | Microbacterium oxydans |
Y321A | the mutation enhances the enzyme activity toward the secondary amines | Microbacterium oxydans |
Y321F | the mutation enhances the enzyme activity toward the secondary amines | Microbacterium oxydans |
Y321I | the mutation enhances the enzyme activity toward the secondary amines and displays an enhanced catalytic efficiency toward 1-(4-methoxybenzyl)-1,2,3,4,5,6,7,8-octahydroisoquinoline | Microbacterium oxydans |
Y321I/M226T | the double mutant acts on (S)-N-(prop-2-yn-1-yl)-2,3-dihydro-1H-inden-1-amine | Microbacterium oxydans |
Y321T | the mutation enhances the enzyme activity toward the secondary amines | Microbacterium oxydans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclohexylamine + O2 + H2O | Microbacterium oxydans | - |
cyclohexanone + NH3 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Microbacterium oxydans | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1R)-1-(4-chlorophenyl)ethan-1-amine + H2O + O2 | - |
Microbacterium oxydans | 1-(4-chlorophenyl)ethanone + NH3 + H2O2 | - |
? | |
(1R)-1-phenylethan-1-amine + H2O + O2 | - |
Microbacterium oxydans | 1-phenylacetaldehyde + NH3 + H2O2 | - |
? | |
(1S)-1-(4-chlorophenyl)ethan-1-amine + H2O + O2 | - |
Microbacterium oxydans | 1-(4-chlorophenyl)ethanone + NH3 + H2O2 | - |
? | |
(1S)-1-phenylethan-1-amine + H2O + O2 | - |
Microbacterium oxydans | 1-phenylacetaldehyde + NH3 + H2O2 | - |
? | |
(1S)-1-phenylpropan-1-amine + H2O + O2 | - |
Microbacterium oxydans | 1-phenylpropan-1-one + NH3 + H2O2 | - |
? | |
(2R)-heptan-2-amine + H2O + O2 | - |
Microbacterium oxydans | heptan-2-one + NH3 + H2O2 | - |
? | |
(2S)-heptan-2-amine + H2O + O2 | - |
Microbacterium oxydans | heptan-2-one + NH3 + H2O2 | - |
? | |
(S)-N-benzyl-1-phenylethylamine + O2 + H2O | - |
Microbacterium oxydans | ? | - |
? | |
cyclohexylamine + O2 + H2O | - |
Microbacterium oxydans | cyclohexanone + NH3 + H2O2 | - |
? | |
additional information | no activitiy towards (R)-N-(prop-2-yn-1-yl)-2, 3-dihydro-1H-inden-1-amine, 1-(4-methoxybenzyl)-1,2,3,4,5,6,7,8-octahydroisoquinoline, (S)-N-methyl-1-phenylethanamine, and 1-methyl-1,2,3,4-tetrahydroisoquinoline | Microbacterium oxydans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CHAO | - |
Microbacterium oxydans |