Application | Comment | Organism |
---|---|---|
analysis | specific and sensitive determination of L-glutamate | Streptomyces violascens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 1 mM AgNO3, complete inhibition | Streptomyces violascens | |
Cu2+ | 1 mM CuCl2, 22.4% inhibition | Streptomyces violascens | |
Hg2+ | 1 mM HgCl2, complete inhibition | Streptomyces violascens | |
N-bromosuccinimide | 1 mM, 77.6% inhibition | Streptomyces violascens | |
PCMB | 1 mM, 42.8% inhibition | Streptomyces violascens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
L-glutamate | pH 5.0 | Streptomyces violascens | |
3.3 | - |
L-glutamate | pH 6.8 | Streptomyces violascens | |
5 | - |
L-His | pH 6.8 | Streptomyces violascens | |
6.7 | - |
L-Gln | pH 6.8 | Streptomyces violascens | |
10 | - |
L-Gln | pH 5.0 | Streptomyces violascens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces violascens | - |
H82-N-SY7 | - |
Streptomyces violascens H82-N-SY7 | - |
H82-N-SY7 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Gln + O2 + H2O | 2.5% of the activity with L-glutamate at pH 5.0, 32.1% of the activity with L-glutamate at pH 6.8 | Streptomyces violascens | 2-oxoglutaric acid 5-amide + NH3 + H2O2 | - |
? | |
L-Gln + O2 + H2O | 2.5% of the activity with L-glutamate at pH 5.0, 32.1% of the activity with L-glutamate at pH 6.8 | Streptomyces violascens H82-N-SY7 | 2-oxoglutaric acid 5-amide + NH3 + H2O2 | - |
? | |
L-glutamate + O2 + H2O | no activity with NADP+, methylene blue, thionine and ferricyanide as electron acceptor | Streptomyces violascens | 2-oxoglutarate + NH3 + H2O2 | - |
? | |
L-glutamate + O2 + H2O | no activity with NADP+, methylene blue, thionine and ferricyanide as electron acceptor | Streptomyces violascens H82-N-SY7 | 2-oxoglutarate + NH3 + H2O2 | - |
? | |
L-His + O2 + H2O | 2.4% of the activity with L-glutamate, 13.1% of the activity with L-glutamate at pH 6.8 | Streptomyces violascens | 2-oxo-3-(1H-imidazol-4yl)-propionic acid + NH3 + H2O2 | - |
? | |
L-His + O2 + H2O | 2.4% of the activity with L-glutamate, 13.1% of the activity with L-glutamate at pH 6.8 | Streptomyces violascens H82-N-SY7 | 2-oxo-3-(1H-imidazol-4yl)-propionic acid + NH3 + H2O2 | - |
? | |
L-Tyr + O2 + H2O | 2.7% of the activity with L-glutamate | Streptomyces violascens | 3-(4-hydroxyphenyl)-2-oxopropionic acid + NH3 + H2O2 | - |
? | |
L-Tyr + O2 + H2O | 2.7% of the activity with L-glutamate | Streptomyces violascens H82-N-SY7 | 3-(4-hydroxyphenyl)-2-oxopropionic acid + NH3 + H2O2 | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Streptomyces violascens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 7 | pH 4.0: about 75% of maximal activity, pH 7.0: about 80% of maximal activity | Streptomyces violascens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 7 | 37°C, 1 h, stable | Streptomyces violascens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | one molecule of noncovalently bound FAD per subunit | Streptomyces violascens |