Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermomyces dupontii |
Protein Variants | Comment | Organism |
---|---|---|
F248Y | mutation markedly increases substrate-binding affinity | Thermomyces dupontii |
General Stability | Organism |
---|---|
enzyme stability decreases following a decrease in protein concentration, and externally added FAD cannot repress the destabilization | Thermomyces dupontii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.71 | - |
D-glutamate | mutant F248Y, pH 7, 60°C | Thermomyces dupontii | |
0.87 | - |
D-Aspartate | mutant F248Y, pH 7, 60°C | Thermomyces dupontii | |
2.16 | - |
D-glutamate | wild-type, pH 7, 60°C | Thermomyces dupontii | |
6.7 | - |
N-Methyl-D-aspartate | mutant F248Y, pH 7, 60°C | Thermomyces dupontii | |
8.77 | - |
D-Aspartate | wild-type, pH 7, 60°C | Thermomyces dupontii | |
25.7 | - |
D-glutamine | mutant F248Y, pH 7, 60°C | Thermomyces dupontii | |
103 | - |
N-Methyl-D-aspartate | wild-type, pH 7, 60°C | Thermomyces dupontii | |
108 | - |
D-glutamine | wild-type, pH 7, 60°C | Thermomyces dupontii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38300 | - |
gel filtration | Thermomyces dupontii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermomyces dupontii | - |
- |
- |
Thermomyces dupontii NBRC 30541 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
46.3 | - |
37°C, pH 7.5 | Thermomyces dupontii |
169 | - |
60°C, pH 7.5 | Thermomyces dupontii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-aspartate + H2O + O2 | - |
Thermomyces dupontii | oxaloacetate + H2O2 + NH3 | - |
? | |
D-aspartate + H2O + O2 | - |
Thermomyces dupontii NBRC 30541 | oxaloacetate + H2O2 + NH3 | - |
? | |
D-glutamate + H2O + O2 | - |
Thermomyces dupontii | 2-oxoglutarate + NH3 + H2O2 | - |
? | |
D-glutamate + H2O + O2 | - |
Thermomyces dupontii NBRC 30541 | 2-oxoglutarate + NH3 + H2O2 | - |
? | |
D-glutamine + H2O + O2 | - |
Thermomyces dupontii | 2-oxoglutaric acid + H2O2 + NH3 | - |
? | |
D-glutamine + H2O + O2 | - |
Thermomyces dupontii NBRC 30541 | 2-oxoglutaric acid + H2O2 + NH3 | - |
? | |
N-methyl-D-aspartate + H2O + O2 | - |
Thermomyces dupontii | oxaloacetate + CH3NH2 + H2O2 | - |
? | |
N-methyl-D-aspartate + H2O + O2 | - |
Thermomyces dupontii NBRC 30541 | oxaloacetate + CH3NH2 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45000, SDS-PAGE | Thermomyces dupontii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Thermomyces dupontii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
51 | - |
T50 value | Thermomyces dupontii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
39.9 | - |
D-glutamine | mutant F248Y, pH 7, 60°C | Thermomyces dupontii | |
50.9 | - |
D-Aspartate | mutant F248Y, pH 7, 60°C | Thermomyces dupontii | |
58.1 | - |
D-glutamate | mutant F248Y, pH 7, 60°C | Thermomyces dupontii | |
68.9 | - |
N-Methyl-D-aspartate | mutant F248Y, pH 7, 60°C | Thermomyces dupontii | |
123 | - |
D-Aspartate | wild-type, pH 7, 60°C | Thermomyces dupontii | |
144 | - |
D-glutamine | wild-type, pH 7, 60°C | Thermomyces dupontii | |
155 | - |
N-Methyl-D-aspartate | wild-type, pH 7, 60°C | Thermomyces dupontii | |
217 | - |
D-glutamate | wild-type, pH 7, 60°C | Thermomyces dupontii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Thermomyces dupontii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | - |
Thermomyces dupontii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | typical absorption spectrum associated with flavoproteins with maxima at 273, 375, and 455 nm | Thermomyces dupontii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
D-glutamine | wild-type, pH 7, 60°C | Thermomyces dupontii | |
1.5 | - |
N-Methyl-D-aspartate | wild-type, pH 7, 60°C | Thermomyces dupontii | |
10.2 | - |
N-Methyl-D-aspartate | mutant F248Y, pH 7, 60°C | Thermomyces dupontii | |
14 | - |
D-Aspartate | wild-type, pH 7, 60°C | Thermomyces dupontii | |
15.5 | - |
D-glutamine | mutant F248Y, pH 7, 60°C | Thermomyces dupontii | |
58.6 | - |
D-Aspartate | mutant F248Y, pH 7, 60°C | Thermomyces dupontii | |
81 | - |
D-glutamate | mutant F248Y, pH 7, 60°C | Thermomyces dupontii | |
100 | - |
D-glutamate | wild-type, pH 7, 60°C | Thermomyces dupontii |