Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phenol | low concentration, magnitude of activation depends on the nature of the acyl-CoA substrate | Yarrowia lipolytica |
Application | Comment | Organism |
---|---|---|
biotechnology | potential depolluting agent by degradation of oils | Yarrowia lipolytica |
biotechnology | several biotechnological applications: production of metabolites, such as citrate, secretion of proteins, degradation of fatty acids | Yarrowia lipolytica |
Cloned (Comment) | Organism |
---|---|
expression of His-tagged enzyme in Escherichia coli | Yarrowia lipolytica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phenol | high concentration, magnitude of inhibition depends on the nature of the acyl-CoA substrate | Yarrowia lipolytica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
decanoyl-CoA | recombinant enzyme | Yarrowia lipolytica | |
0.067 | - |
hexanoyl-CoA | recombinant enzyme | Yarrowia lipolytica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | - |
Yarrowia lipolytica | 5777 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + O2 | Yarrowia lipolytica | involved in beta-oxidation of fatty acids in peroxisomes and glyoxysomes, respectively | trans-2,3-dehydroacyl-CoA + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Yarrowia lipolytica | - |
isoform AOX3 | - |
Yarrowia lipolytica | - |
i.e. Yarrowia lipolytica | - |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli, His-tagged | Yarrowia lipolytica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.95 | - |
recombinant enzyme, purified | Yarrowia lipolytica |
Storage Stability | Organism |
---|---|
-30°C, 35% glycerol (v/v), at least 1 month | Yarrowia lipolytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + O2 | medium-chain-length specific | Yarrowia lipolytica | trans-2,3-dehydroacyl-CoA + H2O2 | - |
? | |
acyl-CoA + O2 | involved in beta-oxidation of fatty acids in peroxisomes and glyoxysomes, respectively | Yarrowia lipolytica | trans-2,3-dehydroacyl-CoA + H2O2 | - |
? | |
decanoyl-CoA + O2 | - |
Yarrowia lipolytica | trans-2-decenoyl-CoA + H2O2 | - |
? | |
furylpropionyl-CoA + O2 | also oxidizes aromatic/heterocyclic ring-substituted chromogenic substrates | Yarrowia lipolytica | furylacryloyl-CoA + H2O2 | - |
? | |
hexanoyl-CoA + O2 | recombinant enzyme | Yarrowia lipolytica | (2E)-hex-2-enoyl-CoA + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AOX3 | - |
Yarrowia lipolytica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | 38 | recombinant enzyme | Yarrowia lipolytica |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 55 | recombinant enzyme, at 5°C, 21% activity, at 55°C, 78% activity | Yarrowia lipolytica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.53 | - |
decanoyl-CoA | recombinant enzyme | Yarrowia lipolytica | |
8.59 | - |
hexanoyl-CoA | recombinant enzyme | Yarrowia lipolytica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
recombinant enzyme | Yarrowia lipolytica |