Crystallization (Comment) | Organism |
---|---|
at 5°C from a 30% saturated ammonium sulfate solution, yellow rod-shaped crystals | Candida tropicalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AgNO3 | - |
Candida tropicalis | |
HgCl2 | - |
Candida tropicalis | |
Mercuric acetate | - |
Candida tropicalis | |
additional information | glutathione protects against inhibition with sulfhydryl reagents | Candida tropicalis | |
p-chloromercuribenzoate | - |
Candida tropicalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
decanoyl-CoA | - |
Candida tropicalis | |
0.024 | - |
lauroyl-CoA | - |
Candida tropicalis | |
0.029 | - |
myristoyl-CoA | - |
Candida tropicalis | |
0.0335 | - |
arachidoyl-CoA | - |
Candida tropicalis | |
0.034 | - |
stearoyl-CoA | - |
Candida tropicalis | |
0.042 | - |
octanoyl-CoA | - |
Candida tropicalis | |
0.046 | - |
oleoyl-CoA | - |
Candida tropicalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
8 * 75000, SDS-PAGE | Candida tropicalis |
600000 | - |
sedimentation equilibrium | Candida tropicalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida tropicalis | - |
pK 233, inducible by growth on alkanes | - |
Purification (Comment) | Organism |
---|---|
- |
Candida tropicalis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
19.13 | - |
- |
Candida tropicalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + O2 | specificity: C4-C20 acyl-CoA | Candida tropicalis | trans-2,3-dehydroacyl-CoA + H2O2 | - |
? | |
arachidoyl-CoA + O2 | - |
Candida tropicalis | 2-trans-eicosenoyl-CoA + H2O2 | - |
? | |
decanoyl-CoA + O2 | - |
Candida tropicalis | trans-2-decenoyl-CoA + H2O2 | - |
? | |
lauroyl-CoA + O2 | - |
Candida tropicalis | trans-2-dodecenoyl-CoA + H2O2 | - |
? | |
myristoyl-CoA + O2 | - |
Candida tropicalis | trans-2-tetradecenoyl-CoA + H2O2 | - |
? | |
octanoyl-CoA + O2 | - |
Candida tropicalis | trans-2-octenoyl-CoA + H2O2 | - |
? | |
oleoyl-CoA + O2 | - |
Candida tropicalis | 2-trans-9-trans-octadecendienoyl-CoA + H2O2 | - |
? | |
palmitoyl-CoA + O2 | - |
Candida tropicalis | 2-trans-hexadecenoyl-CoA + H2O2 | - |
? | |
stearoyl-CoA + O2 | - |
Candida tropicalis | trans-2-octadecenoyl-CoA + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 75000, SDS-PAGE | Candida tropicalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Candida tropicalis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
10 min | Candida tropicalis |
65 | - |
10 min, complete inactivation | Candida tropicalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Candida tropicalis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | 35°C, 60 min | Candida tropicalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein | Candida tropicalis | |
FAD | 8 mol FAD per mol of enzyme, 1 mol FAD per mol of subunit | Candida tropicalis |