Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ammonium sulfate | stimulates activity | Rattus norvegicus | |
CH3COO- NH4+ | stimulates activity | Rattus norvegicus | |
CH3COONa | stimulates activity | Rattus norvegicus | |
Na2S2O5 | stimulates activity | Rattus norvegicus | |
Na2SO4 | stimulates activity | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Top10 cells | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
15-keto prostaglandin F2alpha | 43.06% residual activity at 0.1 mM | Rattus norvegicus | |
benzylidene acetophenone | 21.09% residual activity at 0.5 mM | Rattus norvegicus | |
dicoumarol | 11.45% residual activity at 0.1 mM | Rattus norvegicus | |
indomethacin | 14.22% residual activity at 0.1 mM | Rattus norvegicus | |
additional information | finasteride has no inhibitory effect | Rattus norvegicus | |
quercitrin | 13.14% residual activity at 0.1 mM | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
2-[4-[(E)-(2-oxocyclohexylidene)methyl]phenyl]propanoic acid | at 30°C | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stimulates activity | Rattus norvegicus | |
K+ | stimulates activity | Rattus norvegicus | |
Li+ | stimulates activity | Rattus norvegicus | |
Mg2+ | stimulates activity | Rattus norvegicus | |
Mn2+ | stimulates activity | Rattus norvegicus | |
Na+ | stimulates activity | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
SDS-PAGE | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
2',5'-ADP Sepharose 4B column chromatography, Resource Q column chromatography, and HiLoad Superdex gel filtration | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0054 | - |
cytosol extract | Rattus norvegicus |
0.803 | - |
after 150fold purification | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-[4-[(E)-(2-oxocyclohexylidene)methyl]phenyl]propanoic acid + NADPH | 2-[4-[(E)-(2-oxocyclohexylidene)methyl]phenyl]propanoic acid is also known as CS-670 | Rattus norvegicus | 2-[4-[(2-oxocyclohexyl)methyl]phenyl]propanoic acid + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
bifunctional LTB4 12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase | a beta-NADPH-dependent alkenal/one oxidoreductase which has a role for leukotriene B4 12-hydroxydehydrogenase/15-ketoprostaglandinDELTA13-reductase | Rattus norvegicus |
CS-670 double-bond reductase | - |
Rattus norvegicus |
LTB4 12-HD/PGR | - |
Rattus norvegicus |
NADPH-dependent alkenal/one oxidoreductase | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | dependent | Rattus norvegicus |