Activating Compound | Comment | Organism | Structure |
---|---|---|---|
glutathione | reduced form, reaction rate is maximal, with reduced glutathione | Rattus norvegicus |
General Stability | Organism |
---|---|
dialysis against distilled water, several hours, about 29% loss of activity | Rattus norvegicus |
quite unstable during extensive freezing and thawing | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Iodoacetamide | slight | Rattus norvegicus | |
alpha,alpha'-dipyridyl | slight | Rattus norvegicus | |
Atabrine | slight, FAD reverses inhibition | Rattus norvegicus | |
FeCl3 | - |
Rattus norvegicus | |
NaCN | slight | Rattus norvegicus | |
NADH | inhibits noncompetitively, inhibition not reversed by direct addition of NAD+ | Rattus norvegicus | |
p-chloromercuribenzoate | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
retinal | - |
Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | reaction rate is maximal with Fe2+ | Rattus norvegicus | |
Iron | metalloprotein, approximately 2 mol of iron per mol of enzyme | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
thin layer chromatography, gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
retinal + NAD+ + H2O | Rattus norvegicus | acts on both the all-trans- and 13-cis-form of retinal, all-trans retinal more actively oxidized than 13-cis retinal | retinoate + NADH | - |
ir | |
retinal + NAD+ + H2O | Rattus norvegicus Wistar | acts on both the all-trans- and 13-cis-form of retinal, all-trans retinal more actively oxidized than 13-cis retinal | retinoate + NADH | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
Wistar strain | - |
Rattus norvegicus Wistar | - |
Wistar strain | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
no glycoprotein | - |
Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
retinal + NAD+ + H2O = retinoate + NADH + H+ | acts on both the all-trans- and 13-cis-form of retinal | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
intestinal mucosa | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.12 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
retinal + NAD+ + H2O | acts on both the all-trans- and 13-cis-form of retinal, all-trans retinal more actively oxidized than 13-cis retinal | Rattus norvegicus | retinoate + NADH | - |
ir | |
retinal + NAD+ + H2O | acts on both the all-trans- and 13-cis-form of retinal, all-trans retinal more actively oxidized than 13-cis retinal | Rattus norvegicus Wistar | retinoate + NADH | - |
ir |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at 37°C | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
in 0.1 M sodium phosphate buffer, rather broad pH curve with pH-otimum at approximately pH 7.7 | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | metalloflavoprotein, reaction rate is maximal with FAD | Rattus norvegicus | |
NAD+ | reaction rate is maximal with NAD+, stimulation by NAD+ reversed by direct addition of NADH | Rattus norvegicus |