Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharolobus solfataricus |
SSO1218 expressed in Escherichia coli BL21 (DE3) RIL via isopropyl-b-D-thiogalactopyranoside induction | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
SDS-PAGE | Saccharolobus solfataricus |
54600 | - |
calculated from sequence | Saccharolobus solfataricus |
130000 | - |
native molecular weight using gel filtration | Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methyl-3-oxopropanoate + CoA + H2O + NAD+ | Saccharolobus solfataricus | - |
propanoyl-CoA + HCO3- + NADH | - |
? | |
2-methyl-3-oxopropanoate + CoA + H2O + NAD+ | Saccharolobus solfataricus P2 | - |
propanoyl-CoA + HCO3- + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q97YT9 | - |
- |
Saccharolobus solfataricus P2 | Q97YT9 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
to apparent homogeneity using heat precipitation at 80°C for 20 min, ion exchange chromatography and gel filtration | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methyl-3-oxopropanoate + CoA + H2O + NAD+ | - |
Saccharolobus solfataricus | propanoyl-CoA + HCO3- + NADH | - |
? | |
2-methyl-3-oxopropanoate + CoA + H2O + NAD+ | - |
Saccharolobus solfataricus P2 | propanoyl-CoA + HCO3- + NADH | - |
? | |
malonate semialdehyde + CoA + H2O + NAD+ | - |
Saccharolobus solfataricus | acetyl-CoA + CO2 + NADH | - |
? | |
malonate semialdehyde + CoA + H2O + NAD+ | - |
Saccharolobus solfataricus P2 | acetyl-CoA + CO2 + NADH | - |
? | |
malonate semialdehyde + CoA + NAD+ | - |
Saccharolobus solfataricus | acetyl-CoA + CO2 + NADH | - |
? | |
malonate semialdehyde + CoA + NAD+ | - |
Saccharolobus solfataricus P2 | acetyl-CoA + CO2 + NADH | - |
? | |
methylmalonate semialdehyde + CoA + H2O + NAD+ | - |
Saccharolobus solfataricus | propanoyl-CoA + HCO3- + NADH | - |
? | |
additional information | no activity is observed for the substrates D,L-glyceraldehyde, D,L-glyceraldehyde 3-phosphate and D,L-glycolaldehyde neither with NAD+ nor with NADP+ as cosubstrate | Saccharolobus solfataricus | ? | - |
? | |
additional information | no activity detected in the presence of NADP+ as cosubstrate, no activity with D,L-glyceraldehyde, D,L-glyceraldehyde-3-phosphate and D,L-glycolaldehyde - neither with NAD+ nor with NADP+ as cosubstrate | Saccharolobus solfataricus | ? | - |
? | |
additional information | no activity is observed for the substrates D,L-glyceraldehyde, D,L-glyceraldehyde 3-phosphate and D,L-glycolaldehyde neither with NAD+ nor with NADP+ as cosubstrate | Saccharolobus solfataricus P2 | ? | - |
? | |
additional information | no activity detected in the presence of NADP+ as cosubstrate, no activity with D,L-glyceraldehyde, D,L-glyceraldehyde-3-phosphate and D,L-glycolaldehyde - neither with NAD+ nor with NADP+ as cosubstrate | Saccharolobus solfataricus P2 | ? | - |
? | |
propionaldehyde + CoA + H2O + NAD+ | no physiological substrate | Saccharolobus solfataricus | ? | - |
? | |
propionaldehyde + CoA + NAD+ | highest activity, no physiological substrate for MSDH, it only forms the same thiopropionyl enzyme intermediate as methylmalonate semialdehyde and malonate semialdehyde | Saccharolobus solfataricus | ? + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 50000 | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
methylmalonate semialdehyde dehydrogenase | - |
Saccharolobus solfataricus |
MSDH | - |
Saccharolobus solfataricus |
SSO1218 | - |
Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
methylmalonate semialdehyde | 30°C, pH 8.2 | Saccharolobus solfataricus | |
0.003 | - |
2-Methyl-3-oxopropanoate | pH 8.2, 30°C | Saccharolobus solfataricus | |
0.02 | - |
malonate semialdehyde | 30°C, pH 8.2 | Saccharolobus solfataricus | |
0.02 | - |
malonate semialdehyde | pH 8.2, 30°C | Saccharolobus solfataricus | |
0.03 | - |
propionaldehyde | 30°C, pH 8.2 | Saccharolobus solfataricus | |
0.03 | - |
propionaldehyde | pH 8.2, 30°C | Saccharolobus solfataricus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
CoA | enzyme is strictly CoA-dependent | Saccharolobus solfataricus | |
NAD+ | no activity in the presence of NADP+ as cosubstrate | Saccharolobus solfataricus | |
NAD+ | cosubstrate | Saccharolobus solfataricus |
General Information | Comment | Organism |
---|---|---|
metabolism | for MSDH, a major function in the degradation of branched chain amino acids is proposed which is supported by the high sequence homology with characterized MSDHs from bacteria | Saccharolobus solfataricus |