Cloned (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
C285A | expressed in Escherichia coli, no activity | Rattus norvegicus |
N251E | expressed in Escherichia coli, no activity | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
2-Methyl-3-oxopropanoate | - |
Rattus norvegicus | |
0.0045 | - |
malonate semialdehyde | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | mitochondrial targeting sequence | Rattus norvegicus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55330 | - |
4 * 55330, cDNA sequence | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | acylation of active site cysteine residue Cys-285 by long-chain fatty acyl-CoA esters inactivates the enzyme | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methyl-3-oxopropanoate + CoA + NAD+ | - |
Rattus norvegicus | propanoyl-CoA + CO2 + NADH | - |
ir | |
malonate semialdehyde + CoA + NAD+ | - |
Rattus norvegicus | acetyl-CoA + CO2 + NADH | - |
? | |
p-nitrophenyl acetate + CoA + NAD+ | - |
Rattus norvegicus | p-nitrophenol + acetyl-CoA + NADH | - |
ir |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 55330, cDNA sequence | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
CoA | - |
Homo sapiens | |
CoA | - |
Rattus norvegicus | |
NAD+ | - |
Homo sapiens | |
NAD+ | - |
Rattus norvegicus |