Cloned (Comment) | Organism |
---|---|
expression of splice variant LPMO9A-2 in Pichia pastoris | Gloeophyllum trabeum |
expression of splice variant LpmoA-2 in Pichia pastoris | Gloeophyllum trabeum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | hemicellulose coating has an inhibitory effect on cellulolytic LPMO activity | Gloeophyllum trabeum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25100 | - |
- |
Gloeophyllum trabeum |
25800 | - |
- |
Gloeophyllum trabeum |
33100 | - |
x * 33100, calculated | Gloeophyllum trabeum |
36000 | - |
- |
Gloeophyllum trabeum |
60000 | - |
- |
Gloeophyllum trabeum |
60000 | - |
gel filtration | Gloeophyllum trabeum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gloeophyllum trabeum | - |
- |
- |
Gloeophyllum trabeum | A0A1C9ZMC3 | isoform LPMO9C | - |
Gloeophyllum trabeum | A0A1C9ZMC5 | splice variant LPMO9A-2, which contains a C-terminal domain comprising approximately 55 residues without a predicted function | - |
Gloeophyllum trabeum | A0A1C9ZP88 | splice variant LPMO9A-1 | - |
Gloeophyllum trabeum | A0A1C9ZUN9 | isoform LPMO9D | - |
Gloeophyllum trabeum NBRC 6430 | A0A1C9ZMC3 | isoform LPMO9C | - |
Gloeophyllum trabeum NBRC 6430 | A0A1C9ZMC5 | splice variant LPMO9A-2, which contains a C-terminal domain comprising approximately 55 residues without a predicted function | - |
Gloeophyllum trabeum NBRC 6430 | A0A1C9ZP88 | splice variant LPMO9A-1 | - |
Gloeophyllum trabeum NBRC 6430 | A0A1C9ZUN9 | isoform LPMO9D | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | treatment with EndoH leads to a decrease in molecular mass from 60 kDA to 57 kDa | Gloeophyllum trabeum |
glycoprotein | treatment with EndoH leads to a decrease in molecular mass from 60000 Da to 57000 Da | Gloeophyllum trabeum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxymethyl cellulose + dithiothreitol + O2 | - |
Gloeophyllum trabeum | carboxymethyl cellooligosaccharides + dithiothreitol disulfide + H2O | - |
? | |
carboxymethyl cellulose + dithiothreitol + O2 | reaction is slower with electron donor dithiothreitol than with ascorbate. Reaction is 5- to 10fold slower with carboxymethyl cellulose than with xyloglucan | Gloeophyllum trabeum | carboxymethyl cellooligosaccharides + dithiothreitol disulfide + H2O | - |
? | |
carboxymethyl cellulose + dithiothreitol + O2 | reaction is slower with electron donor dithiothreitol than with ascorbate. Reaction is 5- to 10fold slower with carboxymethyl cellulose than with xyloglucan | Gloeophyllum trabeum NBRC 6430 | carboxymethyl cellooligosaccharides + dithiothreitol disulfide + H2O | - |
? | |
cellulose + dithiothreitol + O2 | - |
Gloeophyllum trabeum | oligosaccharides + dithiothreitol disulfide + H2O | - |
? | |
glucomannan + ascorbate + O2 | - |
Gloeophyllum trabeum | cellooligosaccharides + dehydroascorbate + H2O | - |
? | |
glucomannan + ascorbate + O2 | - |
Gloeophyllum trabeum NBRC 6430 | cellooligosaccharides + dehydroascorbate + H2O | - |
? | |
glucomannan + dithiothreitol + O2 | - |
Gloeophyllum trabeum | cellooligosaccharides + dithiothreitol disulfide + H2O | minor activity on glucomannan | ? | |
additional information | inactive on shorter cellooligosaccharides, such as cellopentaose and cellohexaose, and on arabinoxylan, ivory nut mannan, wheat arabinoxylan, oat spelt xylan, beech wood xylan, and birchwood xylan or mixed linked beta-glucan. Presence of an electron donor is required | Gloeophyllum trabeum | ? | - |
? | |
additional information | isoform LpmoA-2 has broad specificity, cleaving at any position along the beta-glucan backbone of xyloglucan, regardless of substitutions. Inactive on shorter cello-oligosaccharides, such as cellopentaose and cellohexaose, and on arabinoxylan | Gloeophyllum trabeum | ? | - |
? | |
additional information | inactive on shorter cellooligosaccharides, such as cellopentaose and cellohexaose, and on arabinoxylan, ivory nut mannan, wheat arabinoxylan, oat spelt xylan, beech wood xylan, and birchwood xylan or mixed linked beta-glucan. Presence of an electron donor is required | Gloeophyllum trabeum NBRC 6430 | ? | - |
? | |
phosphoric acid swollen cellulose + ascorbate + O2 | - |
Gloeophyllum trabeum | cellooligosaccharides + dehydroascorbate + H2O | - |
? | |
phosphoric acid swollen cellulose + ascorbate + O2 | - |
Gloeophyllum trabeum NBRC 6430 | cellooligosaccharides + dehydroascorbate + H2O | - |
? | |
xyloglucan + ascorbate + O2 | - |
Gloeophyllum trabeum | ? + dehydroascorbate + H2O | releases a broad range of xyloglucan oligosaccharides, cleaving occurs at any position along the beta-glucan backbone of xyloglucan, regardless of substitutions. Dominating products contain a four-glucan backbone (XXXG fragments with various degrees of galactosylation), plus C-1-oxidized and C-4-oxidized cellooligosaccharides | ? | |
xyloglucan + ascorbate + O2 | - |
Gloeophyllum trabeum NBRC 6430 | ? + dehydroascorbate + H2O | releases a broad range of xyloglucan oligosaccharides, cleaving occurs at any position along the beta-glucan backbone of xyloglucan, regardless of substitutions. Dominating products contain a four-glucan backbone (XXXG fragments with various degrees of galactosylation), plus C-1-oxidized and C-4-oxidized cellooligosaccharides | ? | |
xyloglucan + dithiothreitol + O2 | enzyme acts preferentially on free xyloglucan with release of a broad range of xyloglucan oligosaccharides | Gloeophyllum trabeum | xyloglucan oligosaccharides + dithiothreitol disulfide + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33100, calculated | Gloeophyllum trabeum |
? | x * 25100, calculated | Gloeophyllum trabeum |
? | x * 25800, calculated | Gloeophyllum trabeum |
? | x * 36000, splicing variant Lpmo9A-2, calculated | Gloeophyllum trabeum |
? | x * 60000, SDS-PAGE, x * 36000, calculated | Gloeophyllum trabeum |
Synonyms | Comment | Organism |
---|---|---|
LPMO9D | - |
Gloeophyllum trabeum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Gloeophyllum trabeum | calculated | - |
4.6 |
Gloeophyllum trabeum | splicing variant Lpmo9A-2, calculated | - |
4.6 |
Gloeophyllum trabeum | calculated | - |
4.7 |
Gloeophyllum trabeum | calculated | - |
4.9 |
Gloeophyllum trabeum | calculated | - |
5.8 |