Cloned (Comment) | Organism |
---|---|
expression in CHO DG44 cells | Rattus norvegicus |
expression of the truncated enzyme, comprising the catalytic core residues 42-356, in CHO DG44 cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | only the residues 42-356 carrying the monoogenase domain was expressed | Rattus norvegicus |
additional information | large scale production of the enzyme using an automated bioreactor, method optimization and evaluation, overview | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | a copper enzyme, copper binding ratios of recombinant catalytic core, overview | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35040 | - |
MALDI-MS, recombinant enzyme | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
large scale purification of the recombinant truncated enzyme, comprising the catalytic core residues 42-356, from CHO DG44 cells, reconstitution of copper bound to the enzyme | Rattus norvegicus |
two chromatoography steps | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dansyl-D-Tyr-Val-Gly + ascorbate + O2 | - |
Rattus norvegicus | dansyl-D-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PAM | - |
Rattus norvegicus |
peptidylglycine alpha-hydroxylating monooxygenase | - |
Rattus norvegicus |
peptidylglycine monooxygenase | - |
Rattus norvegicus |
PHM | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |