Cloned (Comment) | Organism |
---|---|
heterologous-pathway reconstitution in Nicotiana benthamiana | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
fraxetin + [reduced NADPH-hemoprotein reductase] + O2 | Arabidopsis thaliana | the enzyme is involved in biosynthesis of iron(III)-chelating coumarins in higher plants | sideretin (reduced form) + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9SZ46 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | expressed most strongly in the root epidermal layer under iron-deficient conditions | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
8-methoxypsoralen + [reduced NADPH-hemoprotein reductase] + O2 | - |
Arabidopsis thaliana | 5-hydroxy-8-methoxypsoralen + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
fraxetin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Arabidopsis thaliana | sideretin (reduced form) + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
fraxetin + [reduced NADPH-hemoprotein reductase] + O2 | the enzyme is involved in biosynthesis of iron(III)-chelating coumarins in higher plants | Arabidopsis thaliana | sideretin (reduced form) + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
At4g31940 | - |
Arabidopsis thaliana |
CYP82C4 | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P-450 | a cytochrome P-450 (heme thiolate) enzyme | Arabidopsis thaliana |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | expression is upregulated by iron deficiency. The iron-deficiency-dependent upregulation is mainly confined to roots | up |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in biosynthesis of iron(III)-chelating coumarins in higher plants. Under iron-deficiency conditions, a branch of the phenylpropanoid pathway consisting of feruloyl-CoA 6'-hydroxylase 1, scopoletin 8-hydroxylase, and CYP82C4 is strongly induced, thus leading to the synthesis of scopoletin and its conversion into redox-active fraxetin and sideretin. In the rhizosphere, the redox-active coumarins act in concert with other components of the strategy I iron acquisition machinery by solubilizing and reducing iron from sparingly available sources | Arabidopsis thaliana |