Cloned (Comment) | Organism |
---|---|
expression of active FLAG(TM)-tagged enzyme in Escherichia coli | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0068 | - |
NADPH | pH 7, 37°C, full length 6His-tagged enzyme | Homo sapiens | |
0.0087 | - |
NADPH | pH 7, 37°C, full length 12His-tagged enzyme, FLAG tag removed during affinity purification | Homo sapiens | |
0.1486 | - |
L-kynurenine | pH 7, 37°C, full length 6His-tagged enzyme | Homo sapiens | |
0.153 | - |
L-kynurenine | pH 7, 37°C, full length 12His-tagged enzyme, FLAG tag removed during affinity purification | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
outer mitochondrial membrane | - |
Homo sapiens | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O15229 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-kynurenine + NADPH + H+ + O2 | - |
Homo sapiens | 3-hydroxy-L-kynurenine + NADP+ + H2O | - |
? |
General Information | Comment | Organism |
---|---|---|
drug target | the enzyme is a therapeutic target in several disease states, including Huntington's disease | Homo sapiens |
metabolism | the enzyme is central to the kynurenine pathway of tryptophan metabolism | Homo sapiens |