Cloned (Comment) | Organism |
---|---|
wild-type endothelial enzyme is expressed in insect cells via baculovirus infection | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cyanide | heme-blocker inhibits superoxide formation after pretreatment of the enzyme | Homo sapiens | |
imidazole | heme-blocker inhibits superoxide formation after pretreatment of the enzyme | Homo sapiens | |
Ngamma-nitro-L-arginine methyl ester | only L-isomer, inhibits NO and citrulline production from L-arginine as well as superoxide formation in absence of tetrahydropterin | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | Ca2+/calmodulin is required for citrulline/NO formation and for superoxide formation in absence of tetrahydropterin | Homo sapiens | |
Iron | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
135000 | - |
x * 135000, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type recombinant from insect cells | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
endothelium | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Homo sapiens | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
additional information | enzyme can also Ca2+/calmodulin-dependently produce superoxide in absence of tetrahydropterin and in depletion of L-arginine, which is inhibited by tetrahydropterin, cyanide and imidazole | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 135000, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
about, assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Calmodulin | Ca2+/calmodulin is required for superoxide formation in absence of tetrahydropterin | Homo sapiens | |
NADPH | - |
Homo sapiens |