General Stability | Organism |
---|---|
glutathione stabilizes | Aspergillus niger |
the enzyme is inactivated by treatment with ammonium sulfate or by adsorption on DEAE-cellulose or CM-cellulose, filtration through Sephadex G-25 or dialysis against 0.025 M sodium phosphate buffer, pH 7, containing 1 mM GSH irreversibly inactivates the enzyme | Aspergillus niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Aspergillus niger | |
2,2'-dipyridyl | - |
Aspergillus niger | |
diethyldithiocarbamate | - |
Aspergillus niger | |
heavy metal ions | - |
Aspergillus niger | |
N-ethylmaleimide | - |
Aspergillus niger | |
NaN3 | - |
Aspergillus niger | |
p-chloromercuribenzoate | - |
Aspergillus niger | |
Salicylaldoxime | - |
Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
anthranilic acid | - |
Aspergillus niger | |
0.16 | - |
NADPH | - |
Aspergillus niger |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | inactivates the enzyme | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using protamine sulfate treatment, DEAE-cellulose treatment and alumina C-gamma treatment | Aspergillus niger |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Aspergillus niger | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Aspergillus niger |
Storage Stability | Organism |
---|---|
-20°C, 50% inactivation of the purified enzyme after 2 days | Aspergillus niger |
frozen, mycelium, no appreciable loss of activity after 1 week | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxyanthranilate + NADPH + O2 | no activity | Aspergillus niger | ? | - |
? | |
3-methylanthranilate + NADPH + O2 | no activity | Aspergillus niger | ? + NADP+ + NH3 | - |
? | |
anthranilate + NADPH + O2 | - |
Aspergillus niger | 2,3-dihydroxybenzoate + NADP+ + NH3 | - |
? | |
additional information | no activity with: 3-hydroxyanthranilic acid, benzoic acid, salicylic acid, m-hydroxybenzoic acid, p-hydroxybenzoic acid, m-aminobenzoic acid, p-aminobenzoic acid, methylanthranilate or ethylanthranilate | Aspergillus niger | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
- |
Aspergillus niger |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.5 | at pH 7.5 or pH 9.0 about 60% of activity maximum | Aspergillus niger |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | 2% of the activity when NADPH is replaced by NADH, no effect: FAD, FMN | Aspergillus niger | |
NADPH | absolute requirement for NADPH | Aspergillus niger |