Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 46% inhibition at 0.5 mM , 60% inhibition at 1 mM, 80% inhibition at 3 mM, anthranilic acid protects the enzyme from inhibition | Aspergillus niger | |
2,2'-dipyridyl | 27% inhibition at 0.5 mM, 41% inhibition at 1 mM | Aspergillus niger | |
8-hydroxyquinoline | 23% inhibition at 0.5 mM, 37% inhibition at 1 mM | Aspergillus niger | |
EDTA | 7% inhibition at 1 mM | Aspergillus niger | |
additional information | reversal of 1,10-phenanthroline inhibition by ferric-EDTA, ferrous-EDTA, ferric citrate and cytochrome c | Aspergillus niger | |
Salicylaldoxime | 40% inhibition at 0.5 mM, 52% inhibition at 1 mM | Aspergillus niger |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | the partially purified enzyme is not activated by any metal ion but a considerable decrease in anthranilate hydroxylase activity occurs when the organism is grown on a medium deprived of iron | Aspergillus niger | |
Iron | enzyme-bound iron, participation of Fe in reaction, omission of Fe in the growth medium yields inactive preparation | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
UBC 814 | - |
Aspergillus niger UBC 814 | - |
UBC 814 | - |
Purification (Comment) | Organism |
---|---|
partial, using centrifugation, protamine sulfate treatment, treatment with diethylaminoethyl-cellulose and filtration through a Buchner funnel | Aspergillus niger |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.28 | - |
organism grown on iron-deficient medium, no addition of other constituents | Aspergillus niger |
2.2 | - |
organism grown on iron-deficient medium, addition of 1 mM ferric citrate, preincubation time: 5 min | Aspergillus niger |
4.2 | - |
organism grown on iron-deficient medium, addition of 1 mM ferric citrate, preincubation time: 10 min | Aspergillus niger |
4.6 | - |
organism grown on iron-deficient medium, addition of 1 mM ferric-EDTA | Aspergillus niger |
4.9 | - |
organism grown on iron-deficient medium, addition of 1 mM ferric citrate, preincubation time: 15, 20 or 30 min | Aspergillus niger |
5.6 | - |
organism grown on standard iron-sufficient medium | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
anthranilate + NADPH + O2 | - |
Aspergillus niger | 2,3-dihydroxybenzoate + NADP+ + NH3 | - |
? | |
anthranilate + NADPH + O2 | - |
Aspergillus niger UBC 814 | 2,3-dihydroxybenzoate + NADP+ + NH3 | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
assay at | Aspergillus niger |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Aspergillus niger |