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Literature summary for 1.14.13.1 extracted from

  • Deveryshetty, J.; Phale, P.S.
    Biodegradation of phenanthrene by Alcaligenes sp. strain PPH: partial purification and characterization of 1-hydroxy-2-naphthoic acid hydroxylase (2010), FEMS Microbiol. Lett., 311, 93-101.
    View publication on PubMed
Search for more literature for 1.14.13.1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
salicylate + O2 + NADH + 2 H+ Alcaligenes sp.
-
 catechol + CO2 + H2O + NAD+
-
 ?
salicylate + O2 + NADPH + 2 H+ Alcaligenes sp.
-
 catechol + CO2 + H2O + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Alcaligenes sp.
-
 phenanthrene-degrading
-

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:phenanthrene-grown cell
-
 Alcaligenes sp.
-
culture condition:salicylate-grown cell
-
 Alcaligenes sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0017
-
 salicylate-grown cells, pH 7.5, 30°C Alcaligenes sp.
0.0127
-
 phenanthrene-grown cells, pH 7.5, 30°C Alcaligenes sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
salicylate + O2 + NADH + 2 H+
-
 Alcaligenes sp. catechol + CO2 + H2O + NAD+
-
 ?
salicylate + O2 + NADPH + 2 H+
-
 Alcaligenes sp. catechol + CO2 + H2O + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
salicylate hydroxylase
-
 Alcaligenes sp.
salicylate-1-hydroxylase
-
 Alcaligenes sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Alcaligenes sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 5 min, cell-free extract, in presence of 1-hydroxy-2-naphthoic acid, inactivation Alcaligenes sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Alcaligenes sp.

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Alcaligenes sp.
NADH
-
 Alcaligenes sp.
NADPH
-
 Alcaligenes sp.