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BRENDA support

Literature summary for 1.13.12.7 extracted from

  • Wuensch, D.; Scheve, S.; Weiten, A.; Kalvelage, K.; Rabus, R.
    Luciferase-based determination of ATP/NAD(H) pools in a marine (environmental) bacterium (2022), Microb. Physiol., 32, 122-134 .
    View publication on PubMed

Application

Application Comment Organism
analysis luciferase-based determination of ATP/NAD(H) pools in combination with a microplate reader and the marine model bacterium Phaeobacter inhibens. Grey multiwell plates best balance sensitivity and crosstalk, and optimal incubation times are 5 min and 30 min for the ATP and NAD(H) assay, respectively, together allowing limits of detection of 0.042, 0.470 and 0.710 nM for ATP, NAD+, and NADH, respectively Phaeobacter inhibens

Organism

Organism UniProt Comment Textmining
Phaeobacter inhibens
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Phaeobacter inhibens DSM 17395
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