Activating Compound | Comment | Organism | Structure |
---|---|---|---|
[1,1,3,3-tetramethylguanidine][acetate] | enzyme shows increased activity and increased thermal stability | Photinus pyralis | |
[1,1,3,3-tetramethylguanidine][trichloroacetate] | enzyme shows decreased activity | Photinus pyralis | |
[1,1,3,3-tetramethylguanidine][trifluoroacetate] | enzyme shows unchanged activity | Photinus pyralis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
D-luciferin | pH 7.8, temperature not specified in the publication, 0.125 M [1,1,3,3-tetramethylguanidine][acetate] | Photinus pyralis | |
0.01 | - |
D-luciferin | pH 7.8, temperature not specified in the publication, native enzyme | Photinus pyralis | |
0.02 | - |
D-luciferin | pH 7.8, temperature not specified in the publication, 0.125 M [1,1,3,3-tetramethylguanidine][triflouroacetate] | Photinus pyralis | |
0.06 | - |
ATP | pH 7.8, temperature not specified in the publication, 0.5 M [1,1,3,3-tetramethylguanidine][acetate] | Photinus pyralis | |
0.07 | - |
D-luciferin | pH 7.8, temperature not specified in the publication, 0.125 M [1,1,3,3-tetramethylguanidine][trichloroacetate] | Photinus pyralis | |
0.077 | - |
ATP | pH 7.8, temperature not specified in the publication, 0.125 M [1,1,3,3-tetramethylguanidine][acetate] | Photinus pyralis | |
0.1 | - |
ATP | pH 7.8, temperature not specified in the publication, 0.125 M [1,1,3,3-tetramethylguanidine][triflouroacetate] | Photinus pyralis | |
0.1 | 1 | ATP | pH 7.8, temperature not specified in the publication, native enzyme | Photinus pyralis | |
0.111 | - |
ATP | pH 7.8, temperature not specified in the publication, 0.125 M [1,1,3,3-tetramethylguanidine][trichloroacetate] | Photinus pyralis | |
0.5 | - |
D-luciferin | pH 7.8, temperature not specified in the publication, 0.5 M [1,1,3,3-tetramethylguanidine][triflouroacetate] | Photinus pyralis | |
1 | - |
D-luciferin | pH 7.8, temperature not specified in the publication, 0.5 M [1,1,3,3-tetramethylguanidine][acetate] | Photinus pyralis | |
1.666 | - |
ATP | pH 7.8, temperature not specified in the publication, 0.5 M [1,1,3,3-tetramethylguanidine][triflouroacetate] | Photinus pyralis | |
2.25 | - |
ATP | pH 7.8, temperature not specified in the publication, 0.5 M [1,1,3,3-tetramethylguanidine][trichloroacetate] | Photinus pyralis | |
2.5 | - |
D-luciferin | pH 7.8, temperature not specified in the publication, 0.5 M [1,1,3,3-tetramethylguanidine][trichloroacetate] | Photinus pyralis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Photinus pyralis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-luciferin + ATP + O2 | - |
Photinus pyralis | oxidized D-luciferin + CO2 + H2O + AMP + diphosphate + hv | - |
? |
Synonyms | Comment | Organism |
---|---|---|
luciferase | - |
Photinus pyralis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
in the presence of [1,1,3,3-tetramethylguanidine][trichloroacetate] and [1,1,3,3-tetramethylguanidine][triflouroacetate], enzyme activity reaches to maximum at 20 °C | Photinus pyralis |
25 | - |
native enzyme | Photinus pyralis |
30 | - |
optimum temperature in the presence of [1,1,3,3-tetramethylguanidine][acetate] increases to 30 °C | Photinus pyralis |