Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Photinus pyralis |
Protein Variants | Comment | Organism |
---|---|---|
F14R | improved pH tolerance and stability up to 45°C, no decrease in specific activity relative to the recombinant wild type enzyme | Photinus pyralis |
F14R/L35Q/V182K/I232K/F465R | improved pH tolerance and stability up to 45°C, no decrease in specific activity relative to the recombinant wild type enzyme | Photinus pyralis |
F465R | improved pH tolerance and stability up to 45°C, no decrease in specific activity relative to the recombinant wild type enzyme | Photinus pyralis |
I232K | improved pH tolerance and stability up to 45°C, no decrease in specific activity relative to the recombinant wild type enzyme | Photinus pyralis |
L35Q | improved pH tolerance and stability up to 45°C, no decrease in specific activity relative to the recombinant wild type enzyme | Photinus pyralis |
V182K | improved pH tolerance and stability up to 45°C, no decrease in specific activity relative to the recombinant wild type enzyme | Photinus pyralis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Photinus pyralis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-nitrilotriacetate agarose column chromatography | Photinus pyralis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Photinus luciferin + O2 + ATP | - |
Photinus pyralis | oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Photinus pyralis luciferase | - |
Photinus pyralis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
43 | - |
- |
Photinus pyralis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 45 | - |
Photinus pyralis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
mutant enzymes F14R, L35Q, V182K, I232K and F465R are stable up to 45°C | Photinus pyralis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Photinus pyralis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Photinus pyralis | His10-tagged wild type enzyme | - |
7.2 |